Kimura Kenjiro, Ido Shinichiro, Oyabu Noriaki, Kobayashi Kei, Hirata Yoshiki, Imai Takashi, Yamada Hirofumi
Department of Electronic Science and Engineering, Kyoto University, Katsura, Nishikyo, Kyoto 615-8510, Japan.
J Chem Phys. 2010 May 21;132(19):194705. doi: 10.1063/1.3408289.
Hydration structures at biomolecular surfaces are essential for understanding the mechanisms of the various biofunctions and stability of biomolecules. Here, we demonstrate the measurement of local hydration structures using an atomic force microscopy system equipped with a low-noise deflection sensor. We applied this method to the analysis of the muscovite mica/water interface and succeeded in visualizing a hydration structure that is site-specific on a crystal. Furthermore, at the biomolecule/buffer solution interface, we found surface hydration layers that are more packed than those at the muscovite mica/water interface.
生物分子表面的水化结构对于理解生物分子各种生物功能和稳定性的机制至关重要。在此，我们展示了使用配备低噪声偏转传感器的原子力显微镜系统对局部水化结构的测量。我们将此方法应用于白云母/水界面的分析，并成功可视化了晶体上具有位点特异性的水化结构。此外，在生物分子/缓冲溶液界面，我们发现了比白云母/水界面处排列更紧密的表面水化层。
