用灭活 EV71-Hn2 病毒诱导的单克隆抗体可保护小鼠免受致死性 EV71-Hn2 病毒感染。
Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection.
机构信息
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Fengtai District, Beijing, PR China.
出版信息
Virol J. 2010 May 26;7:106. doi: 10.1186/1743-422X-7-106.
BACKGROUND
Enterovirus 71 (EV71) is a viral pathogen that belongs to the Picornaviridae family, EV71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death.
RESULTS
In this study, several monoclonal antibodies (MAbs) were produced by immunizing mice with the inactived EV71 Henan (Hn2) virus strain. The isolated MAbs were characterised by in vitro neutralizing analysis and peptide ELISA. ELISA assay showed that the neutralizing monoclonal antibody 4E8 specifically reacted with synthetic peptides which contain amino acid 240-250 and 250-260 of EV71 VP1. The in vivo protection assay showed that 4E8 can protect two-day-old BALB/c mice against the lethal challenge of EV71 virus.
CONCLUSION
The MAb 4E8 could be a promising candidate to be humanized and used for treatment of EV71 infection.
背景
肠道病毒 71 型（EV71）是一种病毒病原体，属于小 RNA 病毒科，感染 EV71 的儿童可能会出现严重的神经并发症，导致病情迅速恶化和死亡。
结果
本研究通过用灭活的 EV71 河南（Hn2）病毒株免疫小鼠，产生了几种单克隆抗体（MAb）。分离的 MAb 通过体外中和分析和肽 ELISA 进行了表征。ELISA 检测表明，中和单克隆抗体 4E8 特异性地与包含 EV71 VP1 的 240-250 位和 250-260 位氨基酸的合成肽反应。体内保护试验表明，4E8 可以保护 2 日龄的 BALB/c 小鼠免受 EV71 病毒的致死性攻击。
结论
单克隆抗体 4E8 可能是一种有前途的候选药物，可以进行人源化改造，用于治疗 EV71 感染。
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