Li Linlin, He Yaqing, Yang Hong, Zhu Junping, Xu Xingye, Dong Jie, Zhu Yafang, Jin Qi
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese CDC, No. 6 Rongjing East Street, Yi zhuang, Beijing 100176, China.
J Clin Microbiol. 2005 Aug;43(8):3835-9. doi: 10.1128/JCM.43.8.3835-3839.2005.
The genetic and phylogenetic characteristics of human enterovirus 71 (EV71) and coxsackievirus A16 (CA16) sampled from children with hand, foot, and mouth disease in Shenzhen, People's Republic of China, over a 6-year period (1999 to 2004) were examined with reverse transcription-PCR and DNA sequencing. Out of 147 stool specimens, 60 showed positive signals when screened with EV71- and CA16-specific primers. EV71 was identified in 19 specimens, and CA16 was identified in 41 specimens; coinfection by EV71 and CA16 was not observed. Phylogenetic analysis of all EV71 strains isolated from the mainland Chinese samples established C4 as the predominant genotype. Only one other known strain (3254-TAI-98; AF286531), isolated in Taiwan in 1998, was identified as belonging to genotype C4. Phylogenetic analysis of CA16 strains allowed us to identify three new genetic lineages (A, B, and C), with lineage C recently predominating in Asian countries, such as the People's Republic of China, Malaysia, and Japan. These new observations indicate that CA16 circulating in the People's Republic of China is genetically diverse, and additional surveillance is warranted.
利用逆转录聚合酶链反应（RT-PCR）和DNA测序技术，对1999年至2004年这6年间从中华人民共和国深圳市手足口病患儿中采集的人肠道病毒71型（EV71）和柯萨奇病毒A16型（CA16）的基因和系统发育特征进行了检测。在147份粪便标本中，用EV71和CA16特异性引物筛选时，有60份显示出阳性信号。19份标本中鉴定出EV71，41份标本中鉴定出CA16；未观察到EV71和CA16的混合感染。对从中国大陆样本中分离出的所有EV71毒株进行系统发育分析，确定C4为主要基因型。仅1998年在台湾分离出的另一个已知毒株（3254-TAI-98；AF286531）被鉴定为属于C4基因型。对CA16毒株的系统发育分析使我们能够鉴定出三个新的遗传谱系（A、B和C），其中谱系C最近在亚洲国家如中华人民共和国、马来西亚和日本占主导地位。这些新的观察结果表明，在中华人民共和国流行的CA16在基因上具有多样性，需要进行更多的监测。
