Russel L, Burguet S
Tissue Cell. 1977;9(4):751-66. doi: 10.1016/0040-8166(77)90040-4.
Leydig cells prepared routinely (glutaraldehyde--osmium) for ultrastructural studies are generally found to be lacking in subcellular detail as a result of poor membrane preservation and a dense cytoplasmic matrix. A method modified after that of Karnovsky (1971), utilizing a ferrocyanide--osmium mixture for post-treating glutaraldehyde fixed tissued, was found to yield routinely excellent preservation of Leydig cells. The primary advantages of this method were the enhancement of contrast within the Leydig cell and greatly improved membrane preservation. In addition, the smooth endoplasmic reticulum always appeared as an extensive network of interconnected tubules of uniform diameter; mitochondria, lysosomes, peroxisomes, multivesicular bodies, and Golgi were especially prominent. Glycogen and microfilaments, not readily seen in routine preparations, were found to be abundant in these cells. New observations on the numbers and distributions of subcellular organelles are described and are discussed in relation to their possible role in the steroidogenic process. In view of the greatly improved tissue preservation observed in this study, it is suggested that this treatment be used routinely for preservation of rat Leydig cells.
常规制备用于超微结构研究的（戊二醛-锇）莱迪希细胞，由于膜保存不佳和细胞质基质致密，通常在亚细胞细节方面存在不足。一种在卡诺夫斯基（1971年）方法基础上改进的方法，即利用亚铁氰化物-锇混合物对戊二醛固定的组织进行后处理，被发现能常规地实现对莱迪希细胞的极佳保存。该方法的主要优点是增强了莱迪希细胞内的对比度，并极大地改善了膜的保存。此外，滑面内质网总是呈现为一个由直径均匀的相互连接的小管组成的广泛网络；线粒体、溶酶体、过氧化物酶体、多囊泡体和高尔基体尤为突出。在常规制备中不易看到的糖原和微丝，在这些细胞中被发现大量存在。描述了关于亚细胞器数量和分布的新观察结果，并就它们在类固醇生成过程中可能的作用进行了讨论。鉴于本研究中观察到的组织保存有了极大改善，建议将这种处理常规用于大鼠莱迪希细胞的保存。
