使用锇铁氰化物处理以改善甲状腺滤泡细胞中的溶酶体酸性三聚磷酸酶反应和亚细胞细节。

Use of osmium-ferrocyanide treatment for improved lysosomal acid trimetaphosphatase reaction and subcellular detail in thyroid follicular cells.

作者信息

Payer A F, Battle C L, Peake R L

出版信息

J Histochem Cytochem. 1980 Feb;28(2):183-6. doi: 10.1177/28.2.6243682.

DOI:10.1177/28.2.6243682

Abstract

The histochemical reaction for acid trimetaphosphatase in addition to secondary tissue treatment with an osmium-ferrocyanide mixture was used to study lysosomes and phagolysosomes in the mouse thyroid gland. The osmium-ferrocyanide postfixation enhanced reaction product localization, reduced diffuse reaction, and improved membrane contrast. In addition, the ultrathin tissue sections did not require heavy metal staining, thus eliminating potential stain artifacts due to precipitation. In view of the improved tissue preservation and enzyme localization, it is suggested that osmium-ferrocyanide postfixation be used after the acid trimetaphosphatase method.

摘要

除了用锇 - 亚铁氰化物混合物进行二次组织处理外，酸性三聚偏磷酸酶的组织化学反应被用于研究小鼠甲状腺中的溶酶体和吞噬溶酶体。锇 - 亚铁氰化物后固定增强了反应产物的定位，减少了弥散反应，并改善了膜的对比度。此外，超薄组织切片不需要重金属染色，从而消除了由于沉淀导致的潜在染色假象。鉴于组织保存和酶定位的改善，建议在酸性三聚偏磷酸酶方法之后使用锇 - 亚铁氰化物后固定。