普伐他汀在体外抑制肿瘤坏死因子-α诱导的大鼠血管平滑肌细胞中syndecan-4蛋白的表达

[Pravastatin inhibits the expression of syndecan-4 protein in tumor necrosis factor-alpha-induced rat vascular smooth muscle cells in vitro].

作者信息

Zhang Yu, Ouyang Ping, Luo Ye, Lai Wen-yan, Xu Ding-li

机构信息

Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2010 May;30(5):998-1001.

PMID:20501378

Abstract

OBJECTIVE

To investigate the effect of pravastatin on the proliferation of rat vascular smooth muscle cells (VSMCs) and expression of syndecan-4 protein induced by tumor necrosis factor-alpha (TNF-alpha).

METHODS

VSMCs cultured in vitro were exposed to 20 ng/ml TNF-alpha, 10 micromol/ml pravastatin, 20 micromol/ml pravastatin, 10 micromol/ml pravastatin with 20 ng/ml TNF-alpha, or 20 micromol/ml pravastatin with 20 ng/ml TNF-alpha for 24 h. The proliferation of the VSMCs was determined by non-radioactive MTS/PMS assay and the expression of syndecan-4 protein was detected by Western blotting using anti-syndecan-4 antibody.

RESULTS

Compared to the control group, TNF-alpha at 20 ng/ml significantly stimulated the proliferation of rat VSMCs (P<0.05). Pravastatin alone produced no obvious effect on VSMCs growth (P>0.05), but significantly inhibited TNF-alpha-induced VSMC proliferation (P<0.05). The expression of syndecan-4 protein in the VSMCs was significantly enhanced by 20 ng/ml TNF-alpha (P<0.01). Pravastatin alone did not affect the expression of syndecan-4 protein (P>0.05), but significantly inhibited TNF-alpha-induced enhancement of syndecan-4 protein expression (P<0.01).

CONCLUSION

Pravastatin can inhibit the proliferation and syndean-4 protein expression in rat VSMCs induced by TNF-alpha in vitro.

摘要

目的

研究普伐他汀对肿瘤坏死因子-α（TNF-α）诱导的大鼠血管平滑肌细胞（VSMCs）增殖及Syndecan-4蛋白表达的影响。

方法

将体外培养的VSMCs分别暴露于20 ng/ml TNF-α、10 μmol/ml普伐他汀、20 μmol/ml普伐他汀、10 μmol/ml普伐他汀与20 ng/ml TNF-α或20 μmol/ml普伐他汀与20 ng/ml TNF-α中24小时。采用非放射性MTS/PMS法测定VSMCs的增殖情况，并用抗Syndecan-4抗体通过蛋白质印迹法检测Syndecan-4蛋白的表达。

结果

与对照组相比，20 ng/ml TNF-α显著刺激大鼠VSMCs的增殖（P<0.05）。单独使用普伐他汀对VSMCs生长无明显影响（P>0.05），但显著抑制TNF-α诱导的VSMCs增殖（P<0.05）。20 ng/ml TNF-α显著增强VSMCs中Syndecan-4蛋白的表达（P<0.01）。单独使用普伐他汀不影响Syndecan-4蛋白的表达（P>0.05），但显著抑制TNF-α诱导的Syndecan-4蛋白表达增强（P<0.01）。