[肺成纤维细胞激活在放射性肺损伤中的作用]
[Role of lung fibroblast activation in radiation lung injury].
作者信息
Liu Lai-yu, Ding Yi, Li Qi-sheng, Chen Long-hua, Cai Shao-xi, Guan Jian
机构信息
Department of Respiratory Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2010 May;30(5):1082-4.
OBJECTIVE
To investigate the role of lung fibroblast activation in radiation-induced lung injury.
METHODS
Thirty-five male Wistar rats were exposed to a single-dose 30 Gy irradiation of the right hemithorax or sham right lung irradiation. At 1, 7, 14, 28, 56 or 84 days after the irradiation, the rats were sacrificed for examination of alpha-smooth muscle actin (alpha-SMA) expression in the bilateral lung tissues using immunohistochemistry.
RESULTS
alpha-SMA expression in fibroblast increased significantly in the out-field and in-field lung tissues within 24 h after irradiation after the irradiation (P<0.001).
CONCLUSION
Activation of the lung fibroblasts occurred within 24 h after irradiation and found in ont-field and in-field lung tissues, suggesting that radiation-induced lung injury may not have an obvious latency.
目的
探讨肺成纤维细胞活化在放射性肺损伤中的作用。
方法
35只雄性Wistar大鼠接受右半胸单次30 Gy照射或右肺假照射。照射后1、7、14、28、56或84天，处死大鼠，采用免疫组织化学法检测双侧肺组织中α平滑肌肌动蛋白（α-SMA）的表达。
结果
照射后24 h内，照射野及野内肺组织中，成纤维细胞的α-SMA表达显著增加（P<0.001）。
结论
照射后24 h内肺成纤维细胞发生活化，且在照射野及野内肺组织中均有发现，提示放射性肺损伤可能无明显潜伏期。
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