Franek Edward, Nowak Jacek, Safranow Krzysztof, Adler Grazyna, Bińczak-Kuleta Agnieszka, Ciechanowicz Andrzej, Wiecek Andrzej
Department of Internal Diseases, Endocrinology and Diabetology, Central Clinical Hospital, Ministry for Internal Affairs, Warsaw, Poland.
Pol Arch Med Wewn. 2010 May;120(5):175-80.
Clinical studies have shown either positive or in some other cases negative correlations between leptinemia and bone mineral density (BMD) or bone mineral content (BMC).
The aim of the present study was to assess whether these discrepancies might be associated with the effect of G(-2548)A leptin or A326G and A668G leptin receptor gene polymorphisms on serum leptin concentrations or BMD and BMC.
The study included 72 obese patients (39 women and 33 men, aged 46 +/-8.8 years; body mass index [BMI] >30 kg/m2). In all subjects, serum creatinine, glucose, lipids, leptin, and insulin were determined. Total fat mass (TFM), BMC, and BMD were assessed using dual energy X-ray absorptiometry (Lunar DPX-L).
No significant correlations were observed between body mass composition parameters (TFM, lean mass, BMC) or BMD in relation to genotypes. A positive correlation was found between serum leptin concentration and BMI. An inverse association was observed between leptin concentrations and BMC. Multiple regression analysis showed independent correlations of leptinemia with sex (P <0.001), TFM (P <0.000 001), BMC (P = 0.0001), and the presence of (-2548)A allele of the leptin gene (P <0.05). These parameters together accounted for 83% of variability in serum leptin concentrations.
In obese patients, serum leptin concentration shows an independent inverse correlation with BMD and male sex, but positively with TFM and the presence of -2548A allele of leptin gene. These parameters are responsible for 83% of leptin concentration variability. No correlations between the examined polymorphisms and BMC or BMD were found.
临床研究表明，瘦素血症与骨矿物质密度（BMD）或骨矿物质含量（BMC）之间存在正相关或在其他一些情况下存在负相关。
本研究的目的是评估这些差异是否可能与瘦素基因G（-2548）A或瘦素受体基因A326G和A668G多态性对血清瘦素浓度或BMD和BMC的影响有关。
该研究纳入了72名肥胖患者（39名女性和33名男性，年龄46±8.8岁；体重指数[BMI]>30kg/m²）。测定了所有受试者的血清肌酐、葡萄糖、脂质、瘦素和胰岛素。使用双能X线吸收法（Lunar DPX-L）评估总脂肪量（TFM）、BMC和BMD。
未观察到体重组成参数（TFM、瘦体重、BMC）或BMD与基因型之间存在显著相关性。发现血清瘦素浓度与BMI呈正相关。观察到瘦素浓度与BMC呈负相关。多元回归分析显示，瘦素血症与性别（P<0.001）、TFM（P<0.000001）、BMC（P=0.0001）以及瘦素基因（-2548）A等位基因的存在（P<0.05）存在独立相关性。这些参数共同解释了血清瘦素浓度83%的变异性。
在肥胖患者中，血清瘦素浓度与BMD和男性性别呈独立负相关，但与TFM和瘦素基因-2548A等位基因的存在呈正相关。这些参数导致了83%的瘦素浓度变异性。未发现所检测的多态性与BMC或BMD之间存在相关性。
