Department of Anatomy, Kitasato University School of Allied Health Sciences, Minami-ku, Sagamihara, Japan.
Dev Dyn. 2010 Jun;239(6):1739-47. doi: 10.1002/dvdy.22312.
We cultured the rudimental submandibular gland (SMG) of mice with a non-cell-permeable fluorescent tracer, and observed cell behavior during epithelial branching morphogenesis using confocal time-lapse microscopy. We traced movements of individual cells as shadowgraph movies. Individual epithelial cells migrated dynamically but erratically. The epithelial cleft extended by wiggling and separated a cluster of cells into two buds during branching. We examined the ultrastructure of the clefts in SMG rudiments treated with the laminin peptide A5G77f, which induces epithelial clefting. A short cytoplasmic shelf with a core of microfilaments was found at the deep end of the cleft. We propose that epithelial clefting involves a dynamic movement of cells at the base of the cleft, and the formation of a shelf within a cleft cell. The shelf might form a matrix attachment point at the base of the cleft with a core of microfilaments driving cleft elongation.
我们用非细胞通透性荧光示踪剂培养了小鼠的原始下颌下腺（SMG），并使用共聚焦时相差显微镜观察上皮分支形态发生过程中的细胞行为。我们将单个细胞的运动轨迹追踪为阴影图电影。单个上皮细胞的迁移是动态的，但不稳定。在分支过程中，上皮裂隙通过扭动延伸，并将一群细胞分裂成两个芽。我们检查了用层粘连蛋白肽 A5G77f 处理的 SMG 原基中的裂隙的超微结构，该肽诱导上皮裂隙形成。在裂隙的深部发现了一个带有微丝核心的短细胞质架。我们提出，上皮裂隙形成涉及裂隙底部细胞的动态运动，以及裂隙细胞内的架形成。该架可能在裂隙底部形成一个带有微丝核心的基质附着点，驱动裂隙伸长。
