Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, USA.
J Am Chem Soc. 2010 Jun 23;132(24):8244-5. doi: 10.1021/ja102257v.
Type III protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.
III 型蛋白分泌对于许多革兰氏阴性菌感染宿主细胞至关重要,是新型抗菌药物的一个有吸引力的靶点。在这里,我们描述了一种细菌蛋白效应子羧肽酶 G2(CPG2)报告系统,用于荧光和可见检测鼠伤寒沙门氏菌中的 III 型蛋白分泌。该系统提供了一种通用的方法来测量蛋白表达和分泌,以及一种高通量和定量的分析 III 型蛋白分泌抑制剂的测定方法。