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人类对牙龈卟啉单胞菌(拟杆菌)铁抑制性及其他膜蛋白的免疫球蛋白G抗体反应。

Human immunoglobulin G antibody response to iron-repressible and other membrane proteins of Porphyromonas (Bacteroides) gingivalis.

作者信息

Chen C K, DeNardin A, Dyer D W, Genco R J, Neiders M E

机构信息

Department of Oral Biology, State University of New York, Buffalo 14214.

出版信息

Infect Immun. 1991 Jul;59(7):2427-33. doi: 10.1128/iai.59.7.2427-2433.1991.

Abstract

The human immunoglobulin G (IgG) immune response against Porphyromonas (Bacteroides) gingivalis A7A1-28 iron-repressible membrane proteins (IRMPs) and other membrane proteins was examined by immunoblot analysis. Thirty sera from patients with adult periodontitis and 30 sera from periodontally healthy subjects were included. Iron limitation of P. gingivalis was achieved by growing bacteria in brain heart infusion broth supplemented with protoporphyrin IX and 250 microM alpha, alpha'-dypyridyl, a ferrous iron chelator. Iron-sufficient growth was achieved by growing bacteria in the same medium without alpha, alpha'-dypyridyl. Human sera, in particular those from patients with periodontitis who exhibited high levels of IgG against whole cells of P. gingivalis A7A1-28 in serum in an enzyme-linked immunosorbent assay (ELISA), commonly reacted with five membrane proteins with apparent molecular masses of 80, 67.5, 51, 40.5, and 28 kDa and four IRMPs of 46, 43, 37.5, and 22 kDa. More than 80% of the sera from patients with periodontitis and high levels of IgG against strain A7A1-28 in serum by ELISA reacted with the 46-, 43-, and 37.5-kDa IRMPs, and 40% of these subjects expressed immunoreactivity against the 22-kDa IRMP. Sera from patients with periodontitis and low levels of IgG against strain A7A1-28 in serum by ELISA and sera from periodontally healthy subjects exhibited less immunoreactivity against IRMPs and the five membrane proteins of P. gingivalis. The present study indicates that P. gingivalis IRMPs are immunogenic and that these proteins are expressed in vivo.

摘要

通过免疫印迹分析检测了人类免疫球蛋白G(IgG)针对牙龈卟啉单胞菌(拟杆菌属)A7A1-28铁抑制膜蛋白(IRMPs)及其他膜蛋白的免疫反应。研究纳入了30份来自成人牙周炎患者的血清和30份来自牙周健康受试者的血清。通过在补充了原卟啉IX和250微摩尔α,α'-联吡啶(一种亚铁螯合剂)的脑心浸液肉汤中培养细菌来实现牙龈卟啉单胞菌的铁限制。通过在不含α,α'-联吡啶的相同培养基中培养细菌来实现铁充足条件下的生长。人血清,尤其是那些在酶联免疫吸附测定(ELISA)中血清中针对牙龈卟啉单胞菌A7A1-28全细胞表现出高水平IgG的牙周炎患者血清,通常与5种表观分子量分别为80、67.5、51、40.5和28 kDa的膜蛋白以及4种分子量分别为46、43、37.5和22 kDa的IRMPs发生反应。超过80%的血清中IgG水平高且通过ELISA检测血清中针对A7A1-28菌株呈阳性的牙周炎患者血清与46、43和37.5 kDa的IRMPs发生反应,并且这些受试者中有40%对22 kDa的IRMP表现出免疫反应性。通过ELISA检测血清中IgG水平低且针对A7A1-28菌株呈阴性的牙周炎患者血清以及牙周健康受试者的血清对牙龈卟啉单胞菌的IRMPs和这5种膜蛋白的免疫反应性较低。本研究表明牙龈卟啉单胞菌IRMPs具有免疫原性且这些蛋白在体内表达。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/44b7/258028/6b6ae6e95b9b/iai00043-0213-a.jpg

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