Mouton C, Hammond P G, Slots J, Genco R J
Infect Immun. 1981 Jan;31(1):182-92. doi: 10.1128/iai.31.1.182-192.1981.
An enzyme-linked immunosorbent assay microplate method was used for measuring levels of antibody specific for the oral serotype of Bacteroides asaccharolyticus (Bacteroides gingivalis) in serum samples obtained from umbilical cords, infants, children, periodontally normal adults, and edentulous adults. Serum from patients with various periodontal diseases, including adult periodontitis, localized juvenile periodontitis, generalized juvenile periodontitis, post-localized juvenile periodontitis, and acute necrotizing ulcerative gingivitis, were also studied. A positive correlation between increase in age and increase in both prevalence and level of specific antibody in the G, A, and M classes of immunoglobulins was observed. This indicates that antibodies reactive with oral B. asaccharolyticus found in up to 84% of normal adults are natural antibodies, presumably with a protective role. Among the patient groups, those with adult periodontitis were found to have levels of immunoglobulin G antibodies to oral B. asaccharolyticus that were five times higher than the antibody levels found in control subjects. The levels of IgG antibodies to this organism in the other patient groups were comparable to the levels found in the control group. However, 50% of the individuals in the generalized juvenile periodontitis group had high levels of immunoglobulin G antibodies to B. asaccharolyticus, suggesting heterogeneity with respect to immune response in these patients. These results indicate that antibodies to oral B. asaccharolyticus (B. gingivalis) occur at low levels in most normal children and adults and that the rise in titer of the specific antibodies of each major class of immunoglobulins parallels the ontogenic change in serum levels of that isotype. In contrast, there is a marked increase in titer of immunoglobulin G antibodies to oral B. asaccharolyticus in the group of patients with adult periodontitis and in patients with the generalized form of juvenile periodontitis.
采用酶联免疫吸附测定微孔板法，检测取自脐带血、婴儿、儿童、牙周正常成年人及无牙成年人血清样本中抗解糖嗜胨菌（牙龈拟杆菌）口腔血清型特异性抗体水平。还研究了患有包括成人牙周炎、局限性青少年牙周炎、广泛性青少年牙周炎、局限性青少年牙周炎后期及急性坏死性溃疡性龈炎等各种牙周疾病患者的血清。观察到年龄增长与免疫球蛋白G、A和M类特异性抗体的患病率及水平升高呈正相关。这表明在高达84%的正常成年人中发现的与口腔解糖嗜胨菌反应的抗体是天然抗体，可能具有保护作用。在患者组中，发现成人牙周炎患者针对口腔解糖嗜胨菌的免疫球蛋白G抗体水平比对照组高五倍。其他患者组中针对该菌的IgG抗体水平与对照组相当。然而，广泛性青少年牙周炎组中50%的个体针对解糖嗜胨菌的免疫球蛋白G抗体水平较高，提示这些患者的免疫反应存在异质性。这些结果表明，大多数正常儿童和成年人中针对口腔解糖嗜胨菌（牙龈拟杆菌）的抗体水平较低，且各主要类别的免疫球蛋白特异性抗体滴度的升高与该同种型血清水平的个体发育变化平行。相比之下，成人牙周炎患者组和广泛性青少年牙周炎患者中针对口腔解糖嗜胨菌的免疫球蛋白G抗体滴度显著升高。
