Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
Tissue Eng Part A. 2010 Oct;16(10):3149-57. doi: 10.1089/ten.TEA.2010.0009.
Tissue-engineered blood vessels may provide a solution to the lack of suitable blood vessels for coronary and peripheral vessel bypass grafting. Cyclic strain can be used to provide a more physiological environment that may result in tissue that more closely resembles native artery. In this study, cyclic strain is applied to a collagen-based, tissue-engineered vascular medium. An increased culture time was used to allow the tissue to adhere to the silastic sleeve and to eliminate longitudinal compaction. Cyclic strain improved tissue strength through increased collagen content as well as some radial tissue compaction. Mechanical stimulation promoted a more contractile phenotype and led to a greater contractile response to the vasoconstrictor endothelin-1.
组织工程血管可能为冠状动脉和外周血管旁路移植术提供一种解决方法，即缺乏合适的血管。循环应变可用于提供更接近生理的环境，从而可能导致更类似于天然动脉的组织。在这项研究中，循环应变施加于基于胶原蛋白的组织工程血管培养基上。增加培养时间，使组织能够附着在硅橡胶套管上，并消除纵向压实。循环应变通过增加胶原蛋白含量以及一些径向组织压实来提高组织强度。机械刺激促进了更具收缩性的表型，并导致对血管收缩剂内皮素-1的收缩反应更大。
