跨平台分析全球 microRNA 表达技术。
Cross-platform analysis of global microRNA expression technologies.
机构信息
Environmental Health Sciences and Research Bureau, Health Canada, Ottawa, ON, Canada.
出版信息
BMC Genomics. 2010 May 26;11:330. doi: 10.1186/1471-2164-11-330.
BACKGROUND
Although analysis of microRNAs (miRNAs) by DNA microarrays is gaining in popularity, these new technologies have not been adequately validated. We examined within and between platform reproducibility of four miRNA array technologies alongside TaqMan PCR arrays.
RESULTS
Two distinct pools of reference materials were selected in order to maximize differences in miRNA content. Filtering for miRNA that yielded signal above background revealed 54 miRNA probes (matched by sequence) across all platforms. Using this probeset as well as all probes that were present on an individual platform, within-platform analyses revealed Spearman correlations of >0.9 for most platforms. Comparing between platforms, rank analysis of the log ratios of the two reference pools also revealed high correlation (range 0.663-0.949). Spearman rank correlation and concordance correlation coefficients for miRNA arrays against TaqMan qRT-PCR arrays were similar for all of the technologies. Platform performances were similar to those of previous cross-platform exercises on mRNA and miRNA microarray technologies.
CONCLUSIONS
These data indicate that miRNA microarray platforms generated highly reproducible data and can be recommended for the study of changes in miRNA expression.
背景
尽管 DNA 微阵列分析 microRNAs (miRNAs) 的方法越来越受欢迎，但这些新技术尚未得到充分验证。我们同时使用 TaqMan PCR 阵列，检查了四种 miRNA 阵列技术的平台内和平台间的重现性。
结果
为了最大化 miRNA 含量的差异，选择了两个不同的参考材料池。对产生高于背景信号的 miRNA 进行过滤，揭示了所有平台上 54 个 miRNA 探针（通过序列匹配）。使用这个探针集以及单个平台上存在的所有探针，平台内分析显示大多数平台的 Spearman 相关系数>0.9。在平台之间进行比较时，两个参考池的对数比的秩分析也显示出高度相关性（范围 0.663-0.949）。miRNA 阵列与 TaqMan qRT-PCR 阵列的 Spearman 秩相关系数和一致性相关系数对于所有技术都相似。平台性能与之前关于 mRNA 和 miRNA 微阵列技术的跨平台练习相似。
结论
这些数据表明 miRNA 微阵列平台生成了高度可重复的数据，可推荐用于 miRNA 表达变化的研究。
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