Sesli M, Yeğenoğlu E D
College of Tobacco Expertise, Celal Bayar University, Republic of Turkey, Akhisar, Manisa, Turkey.
Genet Mol Res. 2010 May 25;9(2):966-72. doi: 10.4238/vol9-2gmr783.
Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20, OP-I 1-20, OP-Q 1-20, and OP-J 1-5). Thirty-two of these primers yielded 115 highly polymorphic bands. The mean number of usable bands per primer for all the samples was 3.59. The genetic distance values ranged from 0.1498 to 0.6845, and genetic similarity values varied from 0.8609 to 0.5043. We found that the closest samples based on their genetic distance and similarity values were from Harlak and Sabancilar; the most distant samples were from Bornova and Bademli.
利用RAPD-PCR分析法研究了土耳其野生型橄榄之间的遗传相似性和遗传距离。从马尼萨省和伊兹密尔省的村庄采集了7个野生橄榄树样本。从幼叶中提取基因组DNA,并使用RAPD-PCR分析法生成RAPD标记。对从Operon Technologies公司获得的65个随机引物进行了该分析测试(OP-A 1-20、OP-I 1-20、OP-Q 1-20和OP-J 1-5)。其中32个引物产生了115条高度多态性条带。所有样本每个引物的可用条带平均数为3.59。遗传距离值在0.1498至0.6845之间,遗传相似性值在0.86向9至0.5043之间变化。我们发现,根据遗传距离和相似性值,最接近的样本来自哈拉克和萨班奇拉尔;距离最远的样本来自博尔诺瓦和巴德姆利。