Noormohammadi Z, Samadi-Molayousefi H, Sheidai M
Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Genet Mol Res. 2012 Mar 19;11(1):707-16. doi: 10.4238/2012.March.19.4.
Wild olive (O. europaea ssp cuspidata) plants grow in various regions of Iran and are expected to have considerable genetic diversity due to adaptation to the various environmental conditions. We examined the genetic diversity of four populations of wild olive growing in Hormozgan Province located in southern Iran by using 30 RAPDs and 10 ISSR markers. The mean value of polymorphism for RAPD loci was 73.71%, while the value for ISSR loci was 81.74%. The Keshar population had the highest value of intra-population polymorphism for both RAPD and ISSR loci (66.86 and 62.71%, respectively), while the Tudar population had the lowest values (20.35 and 28.81%, respectively). Similarly, the highest and lowest number of effective alleles, Shannon index and Nei's genetic diversity were also found for these two populations. The highest value of H(pop)/H(sp) within population genetic diversity for RAPD and ISSR loci was found for the Keshar population (H(pop) = 0.85 and H(sp) = 0.90). OPA04-750, OPA13-650 and OPA02-350 RAPD bands were specific for Tudar, Bondon and Keshar populations, respectively, while no specific ISSR bands were observed. Analysis of molecular variance as well as the pairwise F(ST) test showed significant differences for RAPD and ISSR markers among the populations. The NJ and UPGMA trees also separated the wild olive populations from each other, indicating their genetic distinctness. UPGMA clustering of the four wild olive populations placed the Tudar population far from the other populations; Keshar and Bokhoon population samples revealed more similarity and were grouped together. We conclude that there is high genetic diversity among O. europaea ssp cuspidata populations located in southern Iran. We also found RAPD and ISSR markers to be useful molecular tools to discriminate and evaluate genetic variations in wild olive trees.
野生油橄榄（油橄榄欧洲亚种尖叶变种）生长在伊朗的各个地区，由于适应了各种环境条件，预计具有相当大的遗传多样性。我们使用30个随机扩增多态性DNA（RAPD）标记和10个简单序列重复区间（ISSR）标记，研究了生长在伊朗南部霍尔木兹甘省的四个野生油橄榄种群的遗传多样性。RAPD位点的多态性平均值为73.71%，而ISSR位点的多态性值为81.74%。凯沙尔种群在RAPD和ISSR位点上的种群内多态性值最高（分别为66.86%和62.71%），而图达种群的值最低（分别为20.35%和28.81%）。同样，这两个种群的有效等位基因数、香农指数和内氏遗传多样性也分别是最高和最低的。凯沙尔种群在RAPD和ISSR位点的种群遗传多样性中H(pop)/H(sp)值最高（H(pop)=0.85，H(sp)=0.90）。OPA04 - 750、OPA13 - 650和OPA02 - 350的RAPD条带分别对图达、邦东和凯沙尔种群具有特异性，而未观察到特异性的ISSR条带。分子方差分析以及成对F(ST)检验表明，种群间RAPD和ISSR标记存在显著差异。邻接法（NJ）树和非加权组平均法（UPGMA）树也将野生油橄榄种群彼此分开，表明它们的遗传独特性。四个野生油橄榄种群的UPGMA聚类结果显示，图达种群与其他种群相距较远；凯沙尔种群和博洪种群样本显示出更多相似性，并聚在一起。我们得出结论，伊朗南部的油橄榄欧洲亚种尖叶变种种群具有高度的遗传多样性。我们还发现RAPD和ISSR标记是区分和评估野生油橄榄树遗传变异的有用分子工具。
