Sesli M, Yeğenoğlu E D
College of Tobacco Expertise, Turkish Republic, Celal Bayar University, Akhisar, Manisa, Turkey.
Genet Mol Res. 2010 Aug 10;9(3):1550-6. doi: 10.4238/vol9-3gmr866.
We examined genetic relationships among wild and cultivated olives, which is a very important crop in the economy of the Aegean region. We used RAPD analysis to evaluate relationships among and within 22 olive subspecies from Manisa, Mugla and Izmir provinces in Turkey. Twelve of the subspecies were wild and 10 were cultivated olives. Fifty-two primers were used (OP-Q 1-20, OP-I 1-20, OP-F 14-15-16-17, and OP-K 1-8) and 49 polymorphic bands were selected and used for analysis. The dendrogram based on unweighted pair-group cluster analysis using the Sorensen-Dice coefficient of similarity index indicated two major groups, dividing wild olives from cultivated olives. The patterns of genetic relationships among and within the different olives were analyzed by means of analysis of molecular variance. We found significant differences between wild and cultivated olives (Phi(st) = 0.1507; P < 0.001). In order to determine the genetic relationship among wild and cultivated olives, principal coordinate analysis was used to examine the variation among subspecies. The wild and cultivated olives formed two main groups, one on the right side and the other on the left side of the principal coordinates graph, respectively. This was compatible with the results we obtained from analysis of molecular variance.
我们研究了野生橄榄和栽培橄榄之间的遗传关系，橄榄是爱琴海地区经济中非常重要的作物。我们使用随机扩增多态性DNA（RAPD）分析来评估来自土耳其马尼萨、穆拉和伊兹密尔省的22个橄榄亚种之间以及亚种内部的关系。其中12个亚种是野生橄榄，10个是栽培橄榄。使用了52个引物（OP-Q 1 - 20、OP-I 1 - 20、OP-F 14 - 15 - 16 - 17和OP-K 1 - 8），并选择了49个多态性条带用于分析。基于使用索伦森 - 迪西相似性系数的非加权配对组聚类分析构建的树状图显示出两个主要类群，将野生橄榄与栽培橄榄区分开来。通过分子方差分析对不同橄榄之间以及内部的遗传关系模式进行了分析。我们发现野生橄榄和栽培橄榄之间存在显著差异（Phi(st)=0.1507；P<0.001）。为了确定野生橄榄和栽培橄榄之间的遗传关系，使用主坐标分析来检查亚种之间的变异。野生橄榄和栽培橄榄分别在主坐标图的右侧和左侧形成了两个主要类群。这与我们从分子方差分析中获得的结果一致。
