Toygar Mehmet, Demirel Birol, Karslioglu Yildirim, Tugcu Harun, Safali Mukerrem, Senol Ender
Department of Forensic Medicine, Gülhane Military Medical Academy, Kecioren, Ankara, Turkey.
Am J Forensic Med Pathol. 2010 Sep;31(3):227-31. doi: 10.1097/PAF.0b013e3181e214a5.
Distinguishing between injuries resulted from electrical current versus thermal energy is not only a difficult, but also a controversial issue in forensic medicine practice.In this study, an electrical current and a cautery were applied to dorsal skins of 10 rats and biopsies were taken from the injured sites as well as normal skin. In the histologic sections; some planimetric variables such as the perimeter, area, diameter equivalent circle, minimum feret, maximum feret, and the circular form factor of the nuclei located in normal and injured epidermis were measured with the aid of the computer-assisted image analysis.When compared with normal skin, all of the variables -nuclear area, perimeter, diameter equivalent circle, minimum feret, maximum feret, and circular form factor seemed to be decreased both in the electrical current- and cautery-applied skin samples.The differences between the variables measured in normal skin and in electrical- or cautery-applied skin samples were statistically significant (P < 0.05). However none of the variables showed any meaningful differences between the electrical- and cautery-applied areas.It was concluded that the nuclear changes due to electrical current and thermal injury are identical and morphometric analysis seems not to make any further contributions in the differentiating from each other. Therefore, conventional and more established methods for detection of metallization would be more effective.
区分电流损伤与热能损伤不仅在法医学实践中是一个困难且有争议的问题。在本研究中，将电流和烧灼分别作用于10只大鼠的背部皮肤，并从损伤部位以及正常皮肤处取材进行活检。在组织学切片中，借助计算机辅助图像分析测量了一些平面测量变量，如正常和损伤表皮中细胞核的周长、面积、等效圆直径、最小费雷特直径、最大费雷特直径以及圆形形状因子。与正常皮肤相比，在电流作用组和烧灼作用组的皮肤样本中，所有变量——细胞核面积、周长、等效圆直径、最小费雷特直径、最大费雷特直径和圆形形状因子似乎均减小。正常皮肤与电流或烧灼作用皮肤样本中测量的变量之间的差异具有统计学意义（P < 0.05）。然而，在电流作用区和烧灼作用区之间，没有任何变量显示出有意义的差异。得出的结论是，电流损伤和热损伤导致的细胞核变化是相同的，形态计量学分析似乎无法在区分两者方面提供更多帮助。因此，传统且更成熟的检测金属化的方法会更有效。
