Tjellström A, Lindström J, Albrektsson T, Brånemark P I, Hallén O
Acta Otolaryngol. 1978 Mar-Apr;85(3-4):232-42. doi: 10.3109/00016487809111930.
A research project concerning performed autologous ossicles has been going on for more than ten years. Animal experimental studies were followed by a clinical pilot study which showed that bone could be produced in a titanium mould placed in the proximal tibia metaphysis. In the present investigation, in which another five patients were operated on, the experimental design was changed compared to the first pilot study and bone production was improved. In addition to the tibial moulds ten titanium cylinders were placed around a bony bridge prepared in the linea temporalis of the ear to be reconstructed. Nine out of ten of these cylinders contained bone suitable for ossiculoplasty. Histology showed higher osteocyte density and a higher proportion of vital looking osteocytes in the performed grafts compared with a peroperatively sculptured graft of cortical bone. On histochemical investigation the performed graft cells seemed viable at the time of transplantation. The technique for obtaining a preformed autologous ossicle through the titanium cylinders in the temporal bone was easy to perform and the patients did not experience any discomfort. The risk of infection in this area was judged to be small.
一项关于预制自体听小骨的研究项目已经持续了十多年。在动物实验研究之后进行了一项临床试点研究，该研究表明可以在置于胫骨近端干骺端的钛模具中生成骨组织。在本研究中，又有五名患者接受了手术，与首次试点研究相比，实验设计有所改变，骨生成情况得到了改善。除了胫骨模具外，还在准备重建的耳颞线处的骨桥周围放置了十个钛圆柱体。其中十个圆柱体中有九个含有适合听骨成形术的骨组织。组织学显示，与术中雕刻的皮质骨移植物相比，预制移植物中的骨细胞密度更高，存活的骨细胞比例更高。组织化学研究表明，预制移植物细胞在移植时似乎具有活力。通过颞骨中的钛圆柱体获取预制自体听小骨的技术操作简便，患者未感到任何不适。该区域的感染风险被认为较小。
