Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Appl Microbiol Biotechnol. 2010 Aug;87(5):1907-16. doi: 10.1007/s00253-010-2616-1. Epub 2010 May 28.
Cytochrome P450 monooxygenases (P450s) involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium were identified by comprehensive screening of both catalytic potentials and transcriptomic profiling. Functional screening of P. chrysosporium P450s (PcCYPs) revealed that 14 PcCYP species catalyze stepwise conversion of anthracene to anthraquinone via intermediate formation of anthrone. Moreover, transcriptomic profiling explored using a complementary DNA microarray system demonstrated that 12 PcCYPs are up-regulated in response to exogenous addition of anthracene. Among the up-regulated PcCYPs, five species showed catalytic activity against anthracene. Based upon both catalytic and transcriptional properties, these five species are most likely to play major roles in anthracene metabolic processes in vivo. Thus, the combination of functional screening and a microarray system may provide a novel strategy for obtaining a thorough understanding of the catalytic functions and biological impacts of PcCYPs.
细胞色素 P450 单加氧酶（P450s）参与白腐真菌黄孢原毛平革菌对蒽的代谢，通过全面筛选催化潜能和转录组谱鉴定。黄孢原毛平革菌 P450s（PcCYPs）的功能筛选表明，14 种 PcCYP 物种通过蒽酮中间体的形成，催化蒽逐步转化为蒽醌。此外，使用 cDNA 微阵列系统进行的转录组谱探索表明，12 种 PcCYPs 在外源添加蒽时上调。在上调的 PcCYPs 中，有 5 种对蒽具有催化活性。基于催化和转录特性，这 5 种最有可能在体内蒽代谢过程中发挥主要作用。因此，功能筛选和微阵列系统的结合可能为深入了解 PcCYPs 的催化功能和生物学影响提供一种新策略。
