Neurophysiology Laboratory, Division of Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar, 243122, U.P., India.
Biol Trace Elem Res. 2011 Jun;141(1-3):275-82. doi: 10.1007/s12011-010-8733-y. Epub 2010 May 28.
Pineal glands secrets melatonin and various proteins and peptides which has many physiological functions. In keeping with this view, present experiment was conducted to know the effect of buffalo (Bubalus bubalis) pineal proteins (PP) at different dose level on fluoride-induced changes in plasma biochemicals and blood antioxidants enzymes in female rats. For this, we took 30 adult female Wistar rats (133-145 g body weights, BW) and divided into five groups (control, group I; 150 ppm fluoride (F), group II; F+ 50 µg pineal proteins, group III; F+ 100 µg PP, group IV; F+ 200 µg PP, group V). We administered fluoride (150 ppm, drinking water) and F+ pineal proteins at 50, 100, and 200 µg/kg BW, i.p. daily for 21 days. Blood samples were collected at the end of the experiments to estimate plasma glucose, proteins, F, lipid peroxidation (LPO), alkaline phosphatase (ALP), and acetyl cholinesterase (AChE) activity. Red blood cells (RBCs) were separated for analysis of LPO, AChE, catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx), and glutathione reductase (GR) in different groups of animals. Total plasma glucose and protein level did not significantly change in F-treated rats. Plasma ALP and F level were significantly (p < 0.05) high in group II as compared with control and groups III, IV, and V. Administration of PP at different dose level significantly (p < 0.05) reduced the F concentration and ALP activity. Plasma and RBCs AChE activity was significantly (p < 0.05) reduced in F-treated animals as compared with control rats and significantly (p < 0.05) elevated on exogenous administration of PP (groups III and IV). Plasma and RBCs LPO level was significantly (p < 0.05) high in F-alone-treated rats, and PP caused significant (p < 0.05) reduction of LPO in groups IV and V. However, PP treatment in group IV brought better amelioration of F-induced high LPO than in groups III and V. At no dose level, PP-ameliorated F-induced depression of RBCs GSH, CAT, GR, and GPx level. Interestingly, SOD activity was elevated in dose-dependent manner at different dose level of PP in groups III, IV, and V than control and F-administered rats. These findings clearly indicate the beneficial effects of buffalo pineal proteins on fluoride-induced adverse changes in certain plasma biochemical and blood antioxidant systems of rats. It further indicates that PP has dose-dependent ameliorative function against F-induced adverse effects in plasma and blood.
松果腺分泌褪黑素和各种蛋白质和肽，具有许多生理功能。基于这一观点，本实验旨在研究水牛松果腺蛋白（PP）在不同剂量水平下对氟诱导的雌性大鼠血浆生化和血液抗氧化酶变化的影响。为此，我们选取 30 只成年雌性 Wistar 大鼠（体重 133-145g，BW），分为五组（对照组、第 I 组：150ppm 氟（F）、第 II 组：F+50µg 松果腺蛋白、第 III 组：F+100µg PP、第 IV 组：F+200µg PP、第 V 组）。我们给予氟（150ppm，饮用水）和 F+松果腺蛋白 50、100 和 200µg/kg BW，腹腔注射，每天一次，共 21 天。实验结束时采集血液样本，以估计血浆葡萄糖、蛋白质、F、脂质过氧化（LPO）、碱性磷酸酶（ALP）和乙酰胆碱酯酶（AChE）活性。分离红细胞（RBC）分析不同组动物的 LPO、AChE、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽还原酶（GR）。F 处理大鼠的总血浆葡萄糖和蛋白水平无显著变化。与对照组和第 III、IV 和 V 组相比，第 II 组的血浆 ALP 和 F 水平显著（p<0.05）升高。不同剂量水平的 PP 给药显著（p<0.05）降低了 F 浓度和 ALP 活性。与对照组大鼠相比，F 处理动物的血浆和 RBCs AChE 活性显著（p<0.05）降低，而外源性给予 PP（第 III 和 IV 组）则显著（p<0.05）升高。单独用 F 处理的大鼠血浆和 RBCs LPO 水平显著（p<0.05）升高，PP 可显著（p<0.05）降低第 IV 和 V 组的 LPO。然而，与第 III 和 V 组相比，PP 在第 IV 组中对 F 诱导的高 LPO 有更好的改善作用。在任何剂量水平下，PP 均不能改善 F 诱导的 RBCs GSH、CAT、GR 和 GPx 水平降低。有趣的是，与对照组和 F 处理大鼠相比，第 III、IV 和 V 组的 SOD 活性在不同剂量的 PP 下呈剂量依赖性升高。这些发现清楚地表明，水牛松果腺蛋白对氟诱导的大鼠某些血浆生化和血液抗氧化系统的不良变化具有有益作用。进一步表明，PP 对 F 诱导的血浆和血液不良影响具有剂量依赖性的改善作用。
Zotero 插件