Neurophysiology Laboratory, Division of Physiology & Climatology, Indian Veterinary Research Institute, Izatnagar 243122, India.
J Biochem Mol Toxicol. 2012 Jan;26(1):10-5. doi: 10.1002/jbt.20407. Epub 2011 Sep 6.
The objective of this study was to determine the effect of fluoride (F) and arsenic (As) on the activity of acetylcholinesterase (AChE), a critically important nervous system enzyme, and to test the protective role of buffalo epiphyseal (pineal) proteins (BEP) in rats. Arsenic (20 mg/kg BW, intraperitoneally) and F (150 ppm, perorally) were exposed, and BEP was administered intraperitoneally (100 μ g/kg BW) along with F and As to rats for 7 days. As and F exposure significantly (p < 0.05) increased their levels in plasma and decreased the activity of AChE in plasma, RBCs, heart, and brain of rats. Interestingly, As- and F-induced inhibition of AChE activities increased As and F levels in plasma, and organs were significantly (p < 0.05) counteracted by BEP administration. These findings indicate the protective role of buffalo (Bubalus bubalis) epiphyseal proteins on F- and As-induced adverse changes in AChE activity as a candidate biomarker for neurotoxicity in female rats.
本研究旨在确定氟（F）和砷（As）对乙酰胆碱酯酶（AChE）活性的影响，AChE 是一种至关重要的神经系统酶，并测试水牛骨骺（松果体）蛋白（BEP）在大鼠中的保护作用。向大鼠腹腔内注射（20mg/kgBW）砷（As）和经口给予（150ppm）F，同时腹腔内给予（100μg/kgBW）BEP，共 7 天。As 和 F 的暴露显著（p<0.05）增加了它们在血浆中的水平，并降低了大鼠血浆、红细胞、心脏和大脑中 AChE 的活性。有趣的是，As 和 F 诱导的 AChE 活性抑制作用增加了血浆中的 As 和 F 水平，而 BEP 的给予显著（p<0.05）抵消了器官中的这种作用。这些发现表明，水牛（Bubalus bubalis）骨骺蛋白对 F 和 As 诱导的 AChE 活性的不良变化具有保护作用，可作为雌性大鼠神经毒性的候选生物标志物。
