Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.
Int J Environ Res Public Health. 2013 May 2;10(5):1775-85. doi: 10.3390/ijerph10051775.
Fluoride is an environmental and industrial pollutant that affects various organs in humans and animals. The cecal tonsil is an important component of the mucosal immune system and performs important and unique immune functions. In the present study, we investigated the effects of dietary high fluorine on the quantities of IgA+ B cells in the cecal tonsil by immunohistochemistry, and the immunoglobulin A (IgA), immunoglobulin G (IgG) and immunoglobulin M (IgM) contents in the cecal tonsil by ELISA. A total of 280 one-day-old avian broilers were divided into four groups and fed on a corn-soybean basal diet as control diet (fluorine 22.6 mg/kg) or the same diet supplemented with 400, 800 and 1,200 mg/kg fluorine (high fluorine groups I, II and III) in the form of sodium fluoride, respectively, throughout a 42-day experimental period. The results showed that the quantities of IgA+ B cells were lower (p < 0.05 or p < 0.01) and the IgA, IgG, and IgM contents were decreased (p < 0.05 or p < 0.01) in high fluorine groups II and III in comparison with those of control group. It was concluded that dietary fluorine, in the 800-1,200 mg/kg range, could reduce the numbers of the IgA+ B cells and immunoglobulin contents in the cecal tonsil, implying the local mucosal immune function was ultimately impacted in broilers.
氟化物是一种环境和工业污染物，会影响人类和动物的各种器官。盲肠扁桃体是黏膜免疫系统的重要组成部分，具有重要而独特的免疫功能。本研究通过免疫组织化学法检测了日粮高氟对盲肠扁桃体中 IgA+B 细胞数量的影响，通过 ELISA 法检测了盲肠扁桃体中免疫球蛋白 A（IgA）、免疫球蛋白 G（IgG）和免疫球蛋白 M（IgM）的含量。将 280 只 1 日龄肉鸡分为 4 组，分别用玉米-豆粕基础日粮（氟含量 22.6mg/kg）或添加 400、800 和 1200mg/kg 氟（高氟组 I、II 和 III）的氟化钠形式作为对照日粮，整个 42 天实验期。结果表明，与对照组相比，高氟组 II 和 III 的 IgA+B 细胞数量较低（p＜0.05 或 p＜0.01），IgA、IgG 和 IgM 含量降低（p＜0.05 或 p＜0.01）。结论是，日粮氟在 800-1200mg/kg 范围内，可降低盲肠扁桃体中 IgA+B 细胞数量和免疫球蛋白含量，表明肉鸡局部黏膜免疫功能受到影响。
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