Departement de Biochimie, Universite de Montreal, Quebec, Canada.
BMB Rep. 2010 May;43(5):344-8. doi: 10.5483/bmbrep.2010.43.5.344.
Messenger ribonucleoprotein particles (mRNPs) are used to transport mRNAs along neuronal dendrites to their site of translation. Staufen2 is an mRNA-binding protein expressed in the cell bodies and cellular processes of different brain cells. It is notably involved in the transport of dendritic mRNAs along microtubules. Its knockdown expression was shown to change spine morphology and impair synaptic functions. However, the identity of Staufen2-bound mRNAs in brain cells is still completely unknown. As a mean to identify these mRNAs, we immunoprecipitated Staufen2-containing mRNPs from embryonic rat brains and used a genome wide approach to identify Staufen2-associated mRNAs. The genome wide approach identified 1780 mRNAs in Staufen2-containing mRNPs that code for proteins involved in cellular processes such as post-translational protein modifications, RNA metabolism, intracellular transport and translation. These results represent an additional and important step in the characterization of Staufen2- mediated neuronal functions in rat brains.
信使核糖核蛋白颗粒（mRNPs）用于将 mRNA 沿神经元树突运输到其翻译部位。Staufen2 是一种在不同脑细胞的细胞体和细胞过程中表达的 mRNA 结合蛋白。它特别参与沿着微管运输树突状 mRNA。其敲低表达被证明会改变脊柱形态并损害突触功能。然而，脑细胞中 Staufen2 结合的 mRNAs 的身份仍然完全未知。作为鉴定这些 mRNAs 的一种手段，我们从胚胎大鼠脑中免疫沉淀含有 Staufen2 的 mRNPs，并使用全基因组方法鉴定与 Staufen2 相关的 mRNAs。全基因组方法鉴定了 1780 个在含有 Staufen2 的 mRNPs 中编码参与细胞过程的蛋白质的 mRNAs，例如翻译后蛋白质修饰、RNA 代谢、细胞内运输和翻译。这些结果代表了在鉴定大鼠脑中 Staufen2 介导的神经元功能方面的又一个重要步骤。
