[山东结核分枝杆菌临床分离株DNA基因型的分子特征研究]
[Study on molecular characteristics regarding DNA genotype of Mycobacterium tuberculosis clinical strains in Shandong].
作者信息
Deng Yun-feng, Zhang Yan-an, Zheng Jian-li, Jing Hui, Wang Yan, Wang Hai-ying, Ma Xin, Liu Zhi-min
机构信息
Shandong Provincial Chest Hospital, Jinan 250013, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Mar;31(3):321-3.
OBJECTIVE
To establish the molecular characteristics of Mycobacterium tuberculosis and on factors influencing the recent transmission of drug resistant isolates in Shandong.
METHODS
Mycobacterium tuberculosis isolated from active pulmonary tuberculosis patients of 13 counties were genotyped by mycobacterial interspersed repetitive units (MIRU) methods.
RESULTS
12 loci of MIRU were detected in 558 isolates and a total of 143 MIRU patterns were confirmed. 66 isolates had distinct patterns, and 481 (86.2%) strains were in clusters. Shandong cluster included 177 strains with 74.6% of the isolates belonged to Beijing family. The recent transmission index of multi-drug resistance strains was in lower level, comparing to the susceptible strains.
CONCLUSION
Our results showed that the Shandong cluster isolates had capacities of facilitating person-to-person transmission and high level of drug resistance.
目的
确定山东省结核分枝杆菌的分子特征以及影响近期耐药菌株传播的因素。
方法
采用分枝杆菌插入重复单位（MIRU）方法对来自13个县的活动性肺结核患者分离出的结核分枝杆菌进行基因分型。
结果
在558株分离株中检测到12个MIRU位点，共确认了143种MIRU模式。66株分离株具有独特模式，481株（86.2%）菌株呈簇状分布。山东簇包括177株，其中74.6%的分离株属于北京家族。与敏感菌株相比，多重耐药菌株的近期传播指数处于较低水平。
结论
我们的结果表明，山东簇分离株具有人际传播能力和高水平耐药性。
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