[miR-218在胃癌中的表达降低及其意义]
[Reduced expression of miR-218 and its significance in gastric cancer].
作者信息
Gao Cai-ping, Zhang Zhi-yu, Cai Gan-hui, Liu Wen-zhong, Xiao Shu-dong, Lu Hong
机构信息
Department of Gastroenterology, Shanghai Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2010 Apr;32(4):249-52.
OBJECTIVE
To investigate the expression and function of miR-218 in gastric cancer.
METHODS
miR-218 levels were evaluated in 20 non-cardia gastric cancer tissues using TaqMan stem-loop real-time PCR analysis. Pre-miR-218 and anti-miR-218 inhibitor were used to change the miR-218 expression level and examine its effects on cell proliferation, apoptosis, cell cycle and cell invasion.
RESULTS
Comparing with the corresponding normal tissues, miR-218 expression was significantly reduced in the gastric cancer tissue (P < 0.01). Forced expression of miR-218 increased apoptosis in AGS cells. The proportion of apoptosis cells induced by transfection of pre-miR-218 was greater than that induced by control (21.6% vs. 10.4%, P = 0.032). Pre-miR-218 resulted in a significantly decreased cell growth activity (P < 0.01) and cell invasion (P < 0.05) of AGS cells compared with that of the control.
CONCLUSION
miR-218 expression is reduced in gastric cancer. miR-218 may function as a tumor suppressor in gastric carcinoma.
目的
研究miR-218在胃癌中的表达及功能。
方法
采用TaqMan茎环实时定量PCR分析评估20例非贲门胃癌组织中miR-218水平。使用前体miR-218和抗miR-218抑制剂改变miR-218表达水平，并检测其对细胞增殖、凋亡、细胞周期及细胞侵袭的影响。
结果
与相应正常组织相比，胃癌组织中miR-218表达显著降低（P<0.01）。miR-218的强制表达增加了AGS细胞的凋亡。转染前体miR-218诱导的凋亡细胞比例高于对照组（21.6%对10.4%，P=0.032）。与对照组相比，前体miR-218导致AGS细胞的细胞生长活性显著降低（P<0.01）和细胞侵袭能力降低（P<0.05）。
结论
miR-218在胃癌中表达降低。miR-218可能在胃癌中发挥抑癌作用。
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