Qi Min, Li Jian-sheng, Li Yin
Division of Digestive Diseases of the First Clinical Medical School, Zhengzhou University, Zhengzhou 450052, China.
Zhonghua Zhong Liu Za Zhi. 2010 Apr;32(4):283-5.
To investigate the role and clinicopathological significance of RECK and MMP-2 expressions in esophageal squamous cell carcinoma (ESCC).
We collected 57 samples of ESCC tissue and 57 samples of corresponding normal esophageal epithelium tissues in Henan Cancer Hospital. The expression of RECK and MMP-2 was detected with tissue array and immunohistochemistry. Chi-square test and Spearman corrilation analysis were used to analyze the results.
The positive rate of MMP-2 protein expression in cancer tissues was 42.1% (24/57), higher than in normal esophageal epithelia 19.3% (11/57), with a significant difference (chi(2) = 6.968, P < 0.05). For ESCC, a difference was not found in MMP-2 expression among patients with different degree of differentiation and different depth of invasion. The expression levels of RECK in ESCC tissues and normal esophageal epithelia were 36.8% (21/57) and 61.4% (35/57), respectively, with a significant difference (chi(2) = 6.879, P < 0.05). For ESCC, the expression level of RECK was not related to the degree of differentiation, depth of invasion and lymph node metastasis (P > 0.05). For ESCC patients, the expression rates of RECK and MMP-2 were not correlated with age or gender.
The abnormal expression level of the RECK and MMP-2 protein may play an important role in carcinogenesis of esophageal squamous carcinoma. Decreased expression of RECK and increased expression of MMP-2 may be correlated with the esophageal squamous cell carcinoma development, but there is no connection observed between the expression of MMP-2 and RECK.
探讨RECK和基质金属蛋白酶-2（MMP-2）表达在食管鳞状细胞癌（ESCC）中的作用及临床病理意义。
收集河南省肿瘤医院57例ESCC组织样本及57例相应的正常食管上皮组织样本。采用组织芯片和免疫组化法检测RECK和MMP-2的表达。结果采用卡方检验和Spearman相关性分析。
癌组织中MMP-2蛋白表达阳性率为42.1%（24/57），高于正常食管上皮的19.3%（11/57），差异有统计学意义（χ² = 6.968，P < 0.05）。对于ESCC，不同分化程度和不同浸润深度患者的MMP-2表达无差异。ESCC组织和正常食管上皮中RECK的表达水平分别为36.8%（21/57）和61.4%（35/57），差异有统计学意义（χ² = 6.879，P < 0.05）。对于ESCC，RECK的表达水平与分化程度、浸润深度及淋巴结转移无关（P > 0.05）。对于ESCC患者，RECK和MMP-2的表达率与年龄或性别无关。
RECK和MMP-2蛋白的异常表达水平可能在食管鳞状细胞癌的发生中起重要作用。RECK表达降低和MMP-2表达增加可能与食管鳞状细胞癌的发展相关，但未观察到MMP-2与RECK表达之间的联系。
