Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
PLoS One. 2012;7(3):e33517. doi: 10.1371/journal.pone.0033517. Epub 2012 Mar 12.
The reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) down-regulation has been confirmed in numerous human cancers and is clinically associated with metastasis. This study investigates the potential associations of RECK single-nucleotide polymorphisms (SNPs) with hepatocellular carcinoma (HCC) susceptibility and its clinicopathologic characteristics.
METHODOLOGY/PRINCIPAL FINDINGS: A total of 135 HCC cancer patients and 501 cancer-free controls were analyzed for four RECK SNPs (rs10814325, rs16932912, rs11788747, and rs10972727) using real-time PCR and PCR-RFLP genotyping analysis. After adjusting for other co-variants, the individuals carrying RECK promoter rs10814325 inheriting at least one C allele had a 1.85-fold [95% confidence interval (CI), 1.03-3.36] risk of developing HCC compared to TT wild type carriers. The HCC patients, who carried rs11788747 with at least one G allele, had a higher distant metastasis risk than wild type probands.
RECK gene polymorphisms might be a risk factor increasing HCC susceptibility and distant metastasis in Taiwan.
富含半胱氨酸的天冬氨酸蛋白水解酶-激肽释放酶（RECK）下调已在许多人类癌症中得到证实，并与转移有关。本研究探讨了 RECK 单核苷酸多态性（SNP）与肝细胞癌（HCC）易感性及其临床病理特征的潜在相关性。
方法/主要发现：采用实时 PCR 和 PCR-RFLP 基因分型分析，对 135 例 HCC 癌症患者和 501 例无癌症对照者的四个 RECK SNP（rs10814325、rs16932912、rs11788747 和 rs10972727）进行了分析。在调整其他协变量后，与 TT 野生型携带者相比，携带 RECK 启动子 rs10814325 至少携带一个 C 等位基因的个体发生 HCC 的风险增加 1.85 倍（95%置信区间 [CI]，1.03-3.36）。与野生型个体相比，携带 rs11788747 至少携带一个 G 等位基因的 HCC 患者远处转移风险更高。
RECK 基因多态性可能是增加台湾地区 HCC 易感性和远处转移的危险因素。
