Molecular and Cellular Biology Institute, Miguel Hernández University, E-03202 Elche, Alicante, Spain.
Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2273-82. doi: 10.1016/j.fct.2010.05.060. Epub 2010 May 25.
Roots and aerial parts of Cistaceae have been used since ancient times in the Mediterranean cultures for its medicinal properties. In this study, phenolic and tannin content of C. ladanifer and C. populifolius leaves aqueous extracts were determined and their antioxidant and antimicrobial activity were fully studied by several in vitro assays. Their major compounds were identified and quantitated by high-performance liquid chromatography with diode array detection coupled to electrospray ion-trap mass spectrometry. Cytotoxicity on a panel of human cancer cells was also determined. C. populifolius extract was stronger antioxidant than C. ladanifer extract in electron transfer reaction based assays but C. ladanifer extract was more effective to inhibit peroxyl radicals. The major compounds in both extracts were ellagitannins, especially punicalagins derivatives, showing C. populifolius a higher content. C. ladanifer showed noteworthy antibacterial activity against Staphylococcus aureus, whereas C. populifolius was effective against Escherichia coli, with MICs values of 154 and 123 microg/mL, respectively. Last, both extracts showed a notorious capacity to inhibit the proliferation of M220 pancreatic cancer cells and MCF7/HER2 and JIMT-1 breast cancer cells. The leaves of these plants suppose a source for water-soluble ellagitannins-enriched polyphenolic extracts with antioxidant and antimicrobial activities. Their cytotoxic activity against several cancer cells may deserve further attention.
地中海地区的古老文化中，一直以来都将半日花科植物的根和地上部分用于药用。在这项研究中，测定了角果藜和滨藜叶水提物中的酚类和单宁含量，并通过多种体外试验充分研究了其抗氧化和抗菌活性。采用高效液相色谱-二极管阵列检测-电喷雾离子阱质谱联用技术对其主要化合物进行了鉴定和定量分析。还测定了它们对一组人类癌细胞的细胞毒性。在基于电子转移反应的测定中，滨藜叶提取物的抗氧化能力强于角果藜叶提取物，但角果藜叶提取物更有效地抑制过氧自由基。两种提取物中的主要化合物均为鞣花单宁，特别是鞣花酸衍生物，表明滨藜叶中的含量更高。角果藜对金黄色葡萄球菌具有显著的抗菌活性，而滨藜叶对大肠杆菌有效，MIC 值分别为 154 和 123μg/mL。最后，两种提取物均能显著抑制 M220 胰腺癌细胞和 MCF7/HER2 和 JIMT-1 乳腺癌细胞的增殖。这些植物的叶子是富含鞣花单宁的水溶性多酚提取物的来源，具有抗氧化和抗菌活性。它们对多种癌细胞的细胞毒性值得进一步关注。
