Department of Physical and Environmental Sciences, University of Toronto at Scarborough, 1265 Military Trail, Toronto, ON, M1C 1A4, Canada.
Anal Chim Acta. 2010 Jun 11;669(1-2):63-7. doi: 10.1016/j.aca.2010.04.037. Epub 2010 Apr 24.
The detection of trace levels of paraoxon and carbofuran was achieved utilizing differential pulse voltammetry (DPV) on gold disposable electrochemical printed (DEP) chips. The nanostructured gold surface of the chips enables highly sensitive oxidation of the thiocholine (TCh) product even in the absence of costly surface modifications. The inhibition of AChE activity at varying insecticide concentrations was detected with low detection limits of 10 ppb (36 nM) for paraoxon and 8 ppb (18 nM) for carbofuran. Fine-tuning of the experimental conditions will allow for the application of unmodified DEP gold chips for inexpensive on-field detection of AChE inhibition by various insecticides at or below the allowable concentrations set by European and North American regulation standards.
利用金一次性电化学印刷（DEP）芯片上的差分脉冲伏安法（DPV）实现了对痕量对氧磷和呋喃丹的检测。芯片的纳米结构金表面即使没有昂贵的表面修饰，也能使硫代胆碱（TCh）产物高度灵敏地氧化。通过检测不同杀虫剂浓度下 AChE 活性的抑制作用，对氧磷的检测限低至 10ppb（36nM），呋喃丹的检测限低至 8ppb（18nM）。通过微调实验条件，未经修饰的 DEP 金芯片可用于在现场以低廉的成本检测各种杀虫剂对 AChE 的抑制作用，这些杀虫剂的浓度可低至或低于欧洲和北美法规标准规定的允许浓度。
