Xinjiang Institute of Microbiology, Xinjiang Academy of Agricultural Science, Urumqi, Xinjiang, 830091, PR China.
The Key Laboratory for Microbial Resources of Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, PR China.
Int J Syst Evol Microbiol. 2011 Apr;61(Pt 4):974-978. doi: 10.1099/ijs.0.024091-0. Epub 2010 May 28.
A halotolerant actinomycete strain, designated XJEEM 11063(T), was isolated from a salt lake in Xinjiang province, north-western China. Strain XJEEM 11063(T) grew at pH 6.0-8.0 (optimal growth at pH 7.0), between 10 and 40 °C (optimal growth at 28-37 °C) and at salinities of 0-10 % (w/v) NaCl (optimal growth at 0-5 %, w/v). The peptidoglycan type was A4α, and the whole-cell hydrolysates contained glucose, mannose and arabinose. The major fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). MK-9(H(4)) was the predominant menaquinone and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids and three unknown glycolipids. The genomic DNA G+C content was 71.8 mol%. The chemotaxonomic properties supported the affiliation of strain XJEEM 11063(T) to the genus Myceligenerans. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Myceligenerans xiligouense XLG9A10.2(T) (98.3 %) and Myceligenerans crystallogenes DSM 17134(T) (97.0 %). However, it had relatively low values for DNA-DNA relatedness with the above strains (56.0 % and 47.5 %, respectively). Thus, on the basis of the results from this study, a novel species, Myceligenerans halotolerans sp. nov., is proposed. The type strain is XJEEM 11063(T) ( = DSM 21949(T) = CCTCC AA 208063(T)).
一株耐盐放线菌菌株，命名为 XJEEM 11063(T)，从中国西北部新疆的一个盐湖中分离得到。菌株 XJEEM 11063(T)在 pH6.0-8.0（最佳生长 pH7.0）、10-40°C（最佳生长 28-37°C）和 0-10%（w/v）NaCl 盐度（最佳生长 0-5%，w/v）下生长。肽聚糖类型为 A4α，全细胞水解物含有葡萄糖、甘露糖和阿拉伯糖。主要脂肪酸为 anteiso-C(15：0)、iso-C(15：0)和 anteiso-C(17：0)。MK-9(H(4))是主要的menaquinone，极性脂类为双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、两种未知的磷脂和三种未知的糖脂。基因组 DNA G+C 含量为 71.8mol%。化学分类学特性支持菌株 XJEEM 11063(T)属于 Myceligenerans 属。基于 16S rRNA 基因序列的系统发育分析表明，该菌与 Myceligenerans xiligouense XLG9A10.2(T)（98.3%）和 Myceligenerans crystallogenes DSM 17134(T)（97.0%）最为密切相关。然而，与上述菌株的 DNA-DNA 同源性相对较低（分别为 56.0%和 47.5%）。因此，基于本研究的结果，提出了一个新的物种，即 Myceligenerans halotolerans sp. nov.。模式菌株为 XJEEM 11063(T)（=DSM 21949(T)=CCTCC AA 208063(T)）。
