Sarmiento-Vizcaíno Aida, González Verónica, Braña Alfredo F, Molina Axayacatl, Acuña José L, García Luis A, Blanco Gloria
Departamento de Biología Funcional, Área de Microbiología, e Instituto Universitario de Oncología del Principado de Asturias. Universidad de Oviedo, 33006 Oviedo, Spain.
Departamento de Biología de Organismos y Sistemas. Área de Ecología. Universidad de Oviedo, Spain.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1328-1334. doi: 10.1099/ijs.0.000107. Epub 2015 Feb 9.
An actinobacterium strain (M-201(T)) was isolated from a deep-sea scleractinian coral (Fam. Caryophillidae) collected at 1500 m depth in the Avilés Canyon in the Cantabrian Sea, Asturias, Spain. Strain M-201(T) grew at pH 6.0-9.0 (optimum pH 7.0), between 4 and 37 °C (optimum 28 °C) and at salinities of 0.5-10.5% (w/v) NaCl (optimum 0.5-3.0%). The peptidoglycan contained the amino acids Lys, Ala, Thr, Glu and one unknown amino acid component, and belonged to type A4α, and the cell-wall sugars are glucose, mannose and galactose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unknown phosphoglycolipid and seven unknown glycolipids. The predominant menaquinones were MK-9(H4) and MK-9(H6). Major cellular fatty acids were anteiso-C(15 : 0), iso-C(15 : 0) and anteiso-C(17 : 0). The genomic DNA G+C content was 72.4 mol%. The chemotaxonomic properties supported the affiliation of strain M-201(T) to the genus Myceligenerans . Phylogenetic analysis based on 16S rRNA gene sequences revealed that the organism was most closely related to Myceligenerans crystallogenes CD12E2-27(T) (98.2% 16S rRNA gene sequence similarity). However, it had a relatively low DNA-DNA relatedness value with the above strain (48%). The isolate showed antibiotic activity against Escherichia coli , Micrococcus luteus ATCC 14452 and Saccharomyces cerevisiae var. carlsbergensis. To the best of our knowledge, this is the first report of antibiotic production in the genus Myceligenerans . The differences in phenotypic, metabolic, ecological and phylogenetic characteristics justify the proposal of a novel species of the genus Myceligenerans , Myceligenerans cantabricum sp. nov., with M-201(T) ( = CECT 8512(T) = DSM 28392(T)) as the type strain.
从西班牙阿斯图里亚斯坎塔布连海阿维莱斯峡谷1500米深处采集的深海石珊瑚（石珊瑚科）中分离出一株放线菌菌株（M-201(T)）。菌株M-201(T)在pH 6.0 - 9.0（最适pH 7.0）、4至37 °C（最适28 °C）以及盐度为0.5 - 10.5%（w/v）NaCl（最适0.5 - 3.0%）的条件下生长。肽聚糖含有赖氨酸、丙氨酸、苏氨酸、谷氨酸和一种未知氨基酸成分，属于A4α型，细胞壁糖类为葡萄糖、甘露糖和半乳糖。极性脂类为二磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇、一种未知磷酸糖脂和七种未知糖脂。主要的甲基萘醌为MK-9(H4)和MK-9(H6)。主要的细胞脂肪酸为anteiso-C(15 : 0)、iso-C(15 : 0)和anteiso-C(17 : 0)。基因组DNA的G+C含量为72.4 mol%。化学分类学特征支持菌株M-201(T)属于Myceligenerans属。基于16S rRNA基因序列的系统发育分析表明，该菌株与Myceligenerans crystallogenes CD12E2-27(T)关系最为密切（16S rRNA基因序列相似性为98.2%）。然而，它与上述菌株的DNA - DNA相关性值相对较低（48%）。该分离株对大肠杆菌、藤黄微球菌ATCC 14452和卡尔斯伯酵母具有抗菌活性。据我们所知，这是Myceligenerans属中抗生素生产的首次报道。表型、代谢、生态和系统发育特征的差异证明了提出Myceligenerans属的一个新物种——坎塔布连Myceligenerans（Myceligenerans cantabricum sp. nov.）的合理性，以M-201(T)（= CECT 8512(T) = DSM 28392(T)）作为模式菌株。
