School of Bioengineering, Xihua University, Chengdu, 610039, People's Republic of China,
Antonie Van Leeuwenhoek. 2013 Nov;104(5):787-92. doi: 10.1007/s10482-013-9988-z. Epub 2013 Aug 4.
A novel halophilic, filamentous actinomycete, designated TRM 4064(T), was isolated from a hypersaline habitat in Sichuan Province, China. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of strain TRM 4064(T) showed that it was most closely related to Actinopolyspora mortivallis (99.1 % sequence similarity). The sequence similarities between strain TRM 4064(T) and other Actinopolyspora species with validly-published names were <97.0 %. However, it had relatively low mean values for DNA-DNA relatedness with the A. mortivallis DSM 44261(T) (23.2 %). Optimal growth occurred at 37 °C, pH 7.0 and in the presence of 13 % (w/v) NaCl. The whole-cell sugar pattern consists of xylose, glucose, ribose and arabinose. The predominant menaquinones are MK-10(H4) (38.2 %), MK-9(H4) (25.1 %), MK-9(H2) (28.6 %) and MK-8(H4) (7.3 %). The major fatty acids are anteiso-C17:0 (36.9 %) and iso-C17:0 (19.3 %). The diagnostic phospholipids detected were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylinositol (PI) and two unknown phospholipids. The G+C content of the genomic DNA of the type strain is 66.3 mol%. Strain TRM 4064(T) therefore represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora dayingensis sp. nov. is proposed. The type strain is TRM 4064(T) (= KCTC 19979(T) = CCTCC AA 2010010(T)).
一株新的嗜盐、丝状放线菌，命名为 TRM 4064(T)，从中国四川省的一个高盐栖息地中分离出来。基于菌株 TRM 4064(T)的近乎完整的 16S rRNA 基因序列的系统发育分析表明，它与游动放线菌（Actinopolyspora mortivallis）最为密切相关（99.1%的序列相似度）。与具有有效发表名称的其他游动放线菌属种相比，菌株 TRM 4064(T)的序列相似度<97.0%。然而，它与 A. mortivallis DSM 44261(T)的 DNA-DNA 相关度的平均值相对较低（23.2%）。最佳生长条件为 37°C、pH 值 7.0 和 13%（w/v）NaCl。全细胞糖模式由木糖、葡萄糖、核糖和阿拉伯糖组成。主要的类异戊二烯醌是 MK-10(H4)（38.2%）、MK-9(H4)（25.1%）、MK-9(H2)（28.6%）和 MK-8(H4)（7.3%）。主要脂肪酸是anteiso-C17:0（36.9%）和 iso-C17:0（19.3%）。检测到的诊断磷脂有双磷脂酰甘油（DPG）、磷脂酰甘油（PG）、磷脂酰胆碱（PC）、磷脂酰肌醇（PI）和两种未知磷脂。模式菌株的基因组 DNA 的 G+C 含量为 66.3mol%。因此，菌株 TRM 4064(T)代表游动放线菌属的一个新种，建议将其命名为大邑游动放线菌（Actinopolyspora dayingensis）sp. nov.。模式菌株为 TRM 4064(T)=KCTC 19979(T)=CCTCC AA 2010010(T)。
