Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand.
J Exp Biol. 2010 Jun 15;213(Pt 12):2025-30. doi: 10.1242/jeb.041202.
A technique for determining the internal osmotic concentration of a small nematode using a modified nanolitre osmometer is described and used to investigate osmoregulation in the Antarctic nematode Panagrolaimus davidi. This technique enables the osmotic concentration to the measured with an accuracy of +/-12 mmol kg(-1). The pattern of melting in the nematode's different body compartments suggests that it is the osmolality of its pseudocoelomic fluid that is being measured. Panagrolaimus davidi maintains its internal osmotic concentration above that of the external medium and is thus an hyperosmotic regulator. The nematode achieves regulation under hyposmotic stress more rapidly than under hyperosmotic stress.
描述了一种使用改良的纳升级渗透计来测定小型线虫内部渗透浓度的技术，并将其用于研究南极线虫 Panagrolaimus davidi 的渗透调节。该技术可以将渗透压的测量精度提高到 +/-12 mmol kg(-1)。线虫不同体腔部位的融化模式表明，所测量的是其假体腔液的渗透压。Panagrolaimus davidi 将其内部渗透压维持在高于外部介质的水平，因此是一种高渗调节剂。线虫在渗透胁迫下的调节速度比在高渗胁迫下更快。
