Ramløv H, Wharton D A, Wilson P W
Roskilde University Center, Institute of Biology and Chemistry, Denmark.
Cryobiology. 1996 Dec;33(6):607-13. doi: 10.1006/cryo.1996.0064.
The ability of haemolymph from the freezing tolerant weta, Hemideina maori, and supernatant from homogenates of the freezing tolerant nematode Panagrolaimus davidi to inhibit the recrystallization of ice was examined using the "splat freezing" technique and annealing on a cryomicroscope stage. There was no recrystallization inhibition in weta haemolymph or in insect ringer controls. Recrystallization inhibition was present in the nematode supernatant but this was destroyed by heating and was absent in controls. P. davidi survives intracellular freezing and recrystallization inhibition may be important for the survival of this stress.
利用“溅冻”技术并在低温显微镜载物台上进行退火处理，研究了耐寒沙螽（毛利半翅沙螽）血淋巴以及耐寒线虫（大卫食菌线虫）匀浆上清液抑制冰重结晶的能力。沙螽血淋巴或昆虫林格氏液对照中均未出现重结晶抑制现象。线虫上清液中存在重结晶抑制作用，但加热后这种作用被破坏，且对照中不存在该现象。大卫食菌线虫能在细胞内结冰的情况下存活，重结晶抑制作用对于这种应激状态下的生存可能很重要。
