通过定量 PMCA 估计液体和组织中的朊病毒浓度。
Estimating prion concentration in fluids and tissues by quantitative PMCA.
机构信息
Mitchell Center for Alzheimer's Disease and Related Brain Disorders, Department of Neurology, University of Texas Houston Medical School, Houston, Texas, USA.
出版信息
Nat Methods. 2010 Jul;7(7):519-20. doi: 10.1038/nmeth.1465. Epub 2010 May 30.
Abstract
Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.
摘要
朊病毒，这种能引起朊病毒疾病的蛋白质传染性病原体，可以通过蛋白质错误折叠循环扩增（PMCA）技术进行高灵敏度检测。在这里，我们描述了一种定量 PMCA 程序，用于计算生物样本中极低水平朊病毒的浓度。使用此程序，我们确定了感染瘙痒病仓鼠的大脑、脾脏、血液和尿液中错误折叠朊病毒蛋白（PrP(Sc)）的数量。
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