Department of Forensic Odontology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba City 261-0011, Japan.
Int J Legal Med. 2010 Sep;124(5):405-14. doi: 10.1007/s00414-010-0467-z. Epub 2010 May 29.
X-chromosome short tandem repeat (STR) polymorphisms are a useful tool in the fields of human population genetics and personal identification and are indispensable in investigating complex kinship or deficiency cases in circumstances where information on mtDNA or Y-chromosome polymorphisms is unavailable. The purpose of this study was to construct a multiplex polymerase chain reaction (PCR) system capable of analyzing a large number of X-STR loci and establish a 16-X-STR database in the Japanese population We developed two octaplex X-STR systems, one including the DXS7424, GATA172D05, HPRTB, DXS8377, GATA31E08, DXS9895, DXS7423, and DXS981 loci and the other the DXS6803, DXS6789, DXS6800, DXS6809, DXS7133, DXS7132, DXS101, and DXS6807 loci, and conducted a population study in 512 Japanese individuals comprising 339 men and 173 women. A 16-locus multiplex system produced unwanted PCR products due to mixture of the DXS9895 primer with the primers of two other loci. However, a 15-locus multiplex system exclusive of the DXS9895 locus did not. The 15-locus multiplex system amplified the largest number of loci among the X-STR multiplex systems used and afforded a power of discrimination of 0.99999999999997 in women and 0.999999997 in men.
X 染色体短串联重复（STR）多态性是人类群体遗传学和个体识别领域的有用工具，在无法获得 mtDNA 或 Y 染色体多态性信息的情况下，对于调查复杂的亲属关系或缺失案例是不可或缺的。本研究旨在构建一种能够分析大量 X-STR 位点的多重聚合酶链反应（PCR）系统，并在日本人群中建立一个 16-X-STR 数据库。我们开发了两个八重 X-STR 系统，一个包含 DXS7424、GATA172D05、HPRTB、DXS8377、GATA31E08、DXS9895、DXS7423 和 DXS981 位点，另一个包含 DXS6803、DXS6789、DXS6800、DXS6809、DXS7133、DXS7132、DXS101 和 DXS6807 位点，并对 512 名日本个体（包括 339 名男性和 173 名女性）进行了群体研究。由于 DXS9895 引物与另外两个位点的引物混合，16 个位点的多重系统产生了不需要的 PCR 产物。然而，不包含 DXS9895 位点的 15 个位点多重系统则没有。该 15 个位点多重系统在使用的 X-STR 多重系统中扩增了最多的位点，女性的鉴别力为 0.99999999999997，男性为 0.999999997。
