操纵子结合的功能规则及其对蛋白质-DNA 相互作用的影响。
Functional rules for lac repressor-operator associations and implications for protein-DNA interactions.
机构信息
Department of Biochemistry and Biophysics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6059, USA.
出版信息
Protein Sci. 2010 Jun;19(6):1162-72. doi: 10.1002/pro.389.
Abstract
The Lac repressor has been used as a tool to understand protein-DNA recognition for many years. Recent experiments have demonstrated the ability of the Lac repressor to control gene expression in various eukaryotic systems, making the quest for an arsenal of protein-DNA binding partners desirable for potential therapeutic applications. Here, we present the results of the most exhaustive screen of Lac repressor-DNA binding partners to date, resulting in the elucidation of functional rules for Lac-DNA binding. Even within the confines of a single protein-DNA scaffold, modes of binding of different protein-DNA partners are sufficiently diverse so as to prevent elucidation of generalized rules for recognition for a single protein, much less an entire protein family.
摘要
多年来，Lac 阻遏物一直被用作研究蛋白-DNA 识别的工具。最近的实验表明，Lac 阻遏物能够在各种真核系统中控制基因表达，这使得人们渴望获得一系列的蛋白-DNA 结合伙伴，以实现潜在的治疗应用。在这里，我们展示了迄今为止对 Lac 阻遏物-DNA 结合伙伴进行的最详尽的筛选结果，揭示了 Lac-DNA 结合的功能规则。即使在单个蛋白-DNA 支架的范围内，不同蛋白-DNA 结合伙伴的结合模式也足够多样化，以至于无法为单个蛋白甚至整个蛋白家族的识别阐明通用规则。
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