Department of Biochemistry, College of Medicine, Konyang University, Daejon 302-718, Korea.
Oncol Rep. 2010 Jul;24(1):177-81.
Photodynamic therapy has been developed as an alternative therapy of cancer. The aim of this study was to examine whether PDT with hexenyl ester of 5-aminolaevulinic acid (ALA-hx) inhibits the proliferation of the salivary gland adenocarcinoma SGT cells. Cell proliferation was examined by MTT assay. The gene expression of Coproporphyrinogen oxidase (CPO) and ROS production was also examined. Flow cytometry and in vivo Chorioallantoic membrane (CAM) assay was performed. ALA-hx PDT inhibited effectively the proliferation of SGT cells. Treatment of ALA-hx induced CPO mRNA expression and ROS was produced by ALA-hx PDT in SGT cells. Flow cytometry and LDH assay showed that ALA-hx PDT induced necrotic cell death rather than apoptosis in SGT cells. In vivo CAM assay showed that ALA-hx PDT induced tumor destruction by inducing necrosis. These results indicate that ALA-hx PDT effectively inhibits the proliferation of SGT cells by inducing necrosis.
光动力疗法已被开发为癌症的替代疗法。本研究旨在探讨 5-氨基酮戊酸己烯酯（ALA-hx）的 PDT 是否抑制唾液腺癌 SGT 细胞的增殖。通过 MTT 法检测细胞增殖。还检查了原卟啉原氧化酶（CPO）的基因表达和 ROS 的产生。进行了流式细胞术和体内鸡胚尿囊膜（CAM）测定。ALA-hx PDT 有效抑制了 SGT 细胞的增殖。ALA-hx 处理诱导了 SGT 细胞中 CPO mRNA 的表达和 ALA-hx PDT 产生的 ROS。流式细胞术和 LDH 测定表明，ALA-hx PDT 通过诱导坏死导致 SGT 细胞发生坏死性细胞死亡，而不是凋亡。体内 CAM 测定表明，ALA-hx PDT 通过诱导坏死导致肿瘤破坏。这些结果表明，ALA-hx PDT 通过诱导坏死有效抑制 SGT 细胞的增殖。
