Department of Pathology, School of Dentistry, Chosun University, Gwangju 501-759, Korea.
Head Neck. 2010 Sep;32(9):1136-42. doi: 10.1002/hed.21301.
Five-aminolaevulinic acid (ALA) and its derivatives act as precursors of the photosensitizer protoporphyrin IX (PpIX). In this study, the effect of photodynamic therapy (PDT) with hexenyl ester of ALA (ALA-hx) was examined in a human oral squamous cell carcinoma, YD10B cells.
PpIX accumulation and mRNA expression of coproporphyrinogen oxidase (CPO) by ALA and ALA-hx was examined. Cell viability was examined by MTT assay and the molecular mechanism was investigated.
The PpIX synthesis and mRNA expression of CPO was much higher in the cells treated with ALA-hx than ALA. At the concentration that PDT with ALA did not affect cell growth, ALA-hx PDT effectively produced reactive oxygen species (ROS) and suppressed cell growth. Growth inhibition by ALA-hx PDT was due to mitochondrial-dependent apoptosis.
Our results suggest that ALA-hx PDT effectively induced apoptosis of YD-10B cells and can be considered as a therapeutic alternative for oral cancer.
5-氨基酮戊酸（ALA）及其衍生物可作为光敏剂原卟啉 IX（PpIX）的前体。在这项研究中，检查了 5-氨基酮戊酸己烯酯（ALA-hx）的光动力疗法（PDT）对人口腔鳞状细胞癌 YD10B 细胞的作用。
通过 ALA 和 ALA-hx 检测 PpIX 积累和粪卟啉原氧化酶（CPO）的 mRNA 表达。通过 MTT 测定法检查细胞活力，并研究了分子机制。
用 ALA-hx 处理的细胞中 PpIX 的合成和 CPO 的 mRNA 表达要比 ALA 高得多。在 ALA 的 PDT 不会影响细胞生长的浓度下，ALA-hx PDT 有效地产生了活性氧（ROS）并抑制了细胞生长。ALA-hx PDT 引起的生长抑制是由于线粒体依赖性细胞凋亡。
我们的结果表明，ALA-hx PDT 可有效诱导 YD-10B 细胞凋亡，可被视为口腔癌的一种治疗选择。
