Laboratory of Regulatory Biology, Graduate School of Science and Engineering, University of Toyama, 3190-Gofuku, Toyama, Toyama 930-8555, Japan.
J Mol Neurosci. 2011 Oct;45(2):172-6. doi: 10.1007/s12031-010-9390-5. Epub 2010 Jun 1.
Although spice compounds have several pharmacological and biochemical actions such as antioxidant activity, their physiological effects on neuropeptides related to feeding regulation are not well known. The aim of the present study was to identify the pharmacological activities of spice compounds on appetite regulation using a goldfish (Carassius auratus) model with emphasis on the role of neuropeptides. The spice compounds used in this study were curcumin, piperine, and ursolic acid. Goldfish were injected intraperitoneally with test solutions containing each spice or vehicle (including 10% dimethyl sulfoxide in saline), and the changes in food intake were measured every 15 min for 60 min. Among the tested spice compounds, curcumin was found to reduce cumulative food intake and was thus selected for further experiments. Pretreatment with capsaicin, a neurotoxin of afferent nerves, abolished the curcumin-induced decrease of food intake. Curcumin-induced anorexigenic action was also attenuated by intracerebroventricular injection of the corticotropin-releasing hormone (CRH) receptor antagonist α-helical CRH((9-41)). We also examined the expression levels of mRNA for CRH, which is a potent anorexigenic neuropeptide in goldfish, in the diencephalon at 1 h after treatment with curcumin, and found that they were increased. Therefore, the reduction of appetite induced by curcumin treatment in goldfish was suggested to be mediated by the vagal afferent and subsequently through the CRH/CRH receptor pathway.
虽然香料化合物具有几种药理学和生物化学作用，如抗氧化活性，但它们对与摄食调节相关的神经肽的生理作用尚不清楚。本研究的目的是使用金鱼（Carassius auratus）模型确定香料化合物对食欲调节的药理学活性，重点关注神经肽的作用。本研究中使用的香料化合物为姜黄素、胡椒碱和熊果酸。将测试溶液（包括生理盐水 10%二甲基亚砜）经腹腔内注射到金鱼中，每 15 分钟测量一次食物摄入量，持续 60 分钟。在测试的香料化合物中，发现姜黄素可减少累积食物摄入量，因此被选为进一步实验。用辣椒素（传入神经的神经毒素）预处理可消除姜黄素引起的食物摄入量减少。姜黄素引起的厌食作用也被脑室内注射促肾上腺皮质激素释放激素（CRH）受体拮抗剂α-螺旋 CRH（9-41）减弱。我们还检查了在姜黄素处理后 1 小时，下丘脑内 CRH（一种在金鱼中具有强烈厌食作用的神经肽）的 mRNA 表达水平，发现它们增加了。因此，姜黄素处理引起的金鱼食欲下降被认为是通过迷走神经传入纤维介导的，随后通过 CRH/CRH 受体途径。
