Yap Fei-Ling, Cheong Soon-Keng, Ammu Radhakrishnan, Leong Chooi-Fun
Department of Pathology, Faculty of Medicine, International Medical University, Kuala Lumpur, Malaysia.
Malays J Pathol. 2009 Dec;31(2):113-20.
In this study, we evaluated the biological properties of human mesenchymal stem cells transfected (hMSC) with a plasmid vector expressing human cytokine interleukin-12 (IL-12). Surface markers were analysed by immunophenotyping using flow cytometry. Differentiation capability was evaluated towards adipogenesis and osteogenesis. We demonstrated that successfully transfected hMSC retained their surface immunophenotypes and differentiation potential into adipocytes and osteocytes. These results indicate that hMSC may be a suitable vehicle for gene transduction.
