Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Acta Neurol Belg. 2010 Mar;110(1):49-56.
Morphology in diffuse axonal injury (DAI) by lateral head rotation was investigated. SD rats were divided into injury (n=9) and sham (n=3) groups. A device was used to produce lateral rotational acceleration of the rats' heads. At different survival times three rats were killed for light and electron microscopic examination of the brain tissue. Sagittal sections were made from medulla oblongata and immunolabelled for NF68. At post-traumatic 30 min, NF68 immunolabelling showed a small number ofswollen and irregular axons. Ultrastructurally slightly-separated myelin lamellae and disorderly arranged neurofilaments occurred. At 2 and 24 h axonal damage became more severe. Increases in immunolabelled axonal swellings, disconnected axons and axonal retraction bulbs appeared. EM provided evidence of myelin separation, peri-axonal spaces, blank areas in axoplasm, loss of microtubules, peripheral accumulation of mitochondria and clumped neurofilaments for DAI. A tendency was noted for greater labelling with NF68 as axonal damage increased. The disorderly arrangement of NFs occurred at early stage of post-traumatic axonal changes.
对侧头部旋转致弥漫性轴索损伤（DAI）的形态学进行了研究。将 SD 大鼠分为损伤（n=9）和假手术（n=3）组。使用一种装置使大鼠头部产生侧向旋转加速度。在不同的存活时间处死 3 只大鼠，对脑组织进行光镜和电镜检查。从延髓制作矢状切片并用 NF68 进行免疫标记。创伤后 30 分钟，NF68 免疫标记显示少量肿胀和不规则的轴突。超微结构显示轻微分离的髓鞘板层和排列紊乱的神经丝。在 2 小时和 24 小时，轴突损伤变得更加严重。免疫标记的轴突肿胀、断开的轴突和轴突回缩球增加。EM 为 DAI 提供了髓鞘分离、轴周间隙、轴浆空白区、微管丢失、线粒体外周堆积和神经丝聚集的证据。随着轴突损伤的增加，NF68 的标记呈增加趋势。NF 的紊乱排列发生在创伤后轴突变化的早期。
