一种采用紫外检测的高效液相色谱法测定人血清中加巴喷丁的柱前衍生化新方法。
A new pre-column derivatization method for determination of gabapentin in human serum by HPLC using UV detection.
作者信息
Amini Mohsen, Rouini Mohammad-Reza, Asad-Paskeh Alireza, Shafiee Abbas
机构信息
Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences.
出版信息
J Chromatogr Sci. 2010 May-Jun;48(5):358-61. doi: 10.1093/chromsci/48.5.358.
A high-performance liquid chromatography method using UV detection for determination of gabapentin in human serum has been developed. Serum was deproteinized with acetonitrile, and supernatant was evaporated. Derivatization was performed using 4-nitrophenylisothiocyanate, and separation was achieved on a C(18) column with detection at 335 nm. The method was linear in the 0.12-6.0 microg/mL range. No interference was found from endogenous amino acids. The method is suitable for routine therapeutic drug monitoring and for pharmacokinetic studies.
已开发出一种采用紫外检测的高效液相色谱法，用于测定人血清中的加巴喷丁。血清用乙腈进行脱蛋白处理，然后将上清液蒸发。使用异硫氰酸4-硝基苯酯进行衍生化反应，并在C(18)柱上进行分离，检测波长为335 nm。该方法在0.12 - 6.0微克/毫升范围内呈线性。未发现内源性氨基酸的干扰。该方法适用于常规治疗药物监测和药代动力学研究。
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