A new pre-column derivatization method for determination of gabapentin in human serum by HPLC using UV detection.

作者信息

Amini Mohsen, Rouini Mohammad-Reza, Asad-Paskeh Alireza, Shafiee Abbas

机构信息

Department of Medicinal Chemistry and Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences.

出版信息

J Chromatogr Sci. 2010 May-Jun;48(5):358-61. doi: 10.1093/chromsci/48.5.358.

A high-performance liquid chromatography method using UV detection for determination of gabapentin in human serum has been developed. Serum was deproteinized with acetonitrile, and supernatant was evaporated. Derivatization was performed using 4-nitrophenylisothiocyanate, and separation was achieved on a C(18) column with detection at 335 nm. The method was linear in the 0.12-6.0 microg/mL range. No interference was found from endogenous amino acids. The method is suitable for routine therapeutic drug monitoring and for pharmacokinetic studies.