Li Yan, Shirasaka Yoshiyuki, Langguth Peter, Tamai Ikumi
Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamasaki, Noda, Chiba 278-8510, Japan.
J Chromatogr Sci. 2010 May-Jun;48(5):367-70. doi: 10.1093/chromsci/48.5.367.
The aim of this study was to develop and validate an assay based on liquid chromatography-tandem mass spectrometry to quantitate talinolol in rat plasma. After a simple protein precipitation step, separation was performed by reversed-phase liquid chromatography using gradient elution with acetonitrile-water-formic acid. Electrospray ionization in the positive ion mode with multiple reaction monitoring was used to analyze talinolol employing propranolol as internal standard. The calibration curve for talinolol was linear over the concentration range 1-250 ng/mL with a correlation coefficient > 0.995. The method was sensitive (limit of quantitation, 1 ng/mL) and had acceptable accuracy (85-115% of true values) and precision (intra- and inter-assay CV < 15%). Recovery of talinolol at concentrations of 1, 25, and 250 ng/mL was >78%. The method was successfully applied to the determination of the oral pharmacokinetics of talinolol in rat.
