Xu Miao, Su Wei, Xu Qiu-ping, Huang Wei-dong
Department of Critical Care Medicine, Sir Run Run Shaw Hospital, Medical College of Zhejiang University and Sir Run Run Shaw Institute of Clinical Medicine, Hangzhou, China.
Chin J Traumatol. 2010 Jun 1;13(3):158-62.
To explore the effects of Xingnaojing injection on cerebral edema and blood-brain barrier (BBB) in rats following traumatic brain injury (TBI).
A total of 108 adult male Sprague-Dawley rats were used as subjects and randomly assigned to three groups: sham-operation, TBI and Xingnaojing injection groups (10 ml/kg/d, intraperitoneal injection). TBI in rats was set up by the improved device of Feeney's weight-dropping model with the impact of 600 g.cm. Brain water content and BBB permeability expressed as Evans blue content were measured at 1, 3, 5 and 7 days after surgery.
In sham-operation group, brain water content and Evans blue content in brain tissue were 78.97%+/-1.22% and 5.13 microgram+/-0.71 microgram. Following TBI, water content in brain tissue was increased significantly at 1, 3, 5 and 7 days (83.49%+/-0.54%, 82.74%+/-0.72%, 80.22%+/-0.68%, 79.21%+/-0.60%), being significantly higher than that in sham operation group (P less than 0.05). Evans blue content was increased in TBI group (16.54 microgram+/-0.60 microgram, 14.92 microgram+/-0.71 microgram, 12.44 microgram+/-0.92 microgram, 10.14 microgram+/-0.52 microgram) as compared with sham-operation group(P less than 0.05). After treatment with Xingnaojing injection, brain water content decreased as compared with TBI group (81.91%+/-1.04%, 80.38%+/-0.72%, 79.54%+/-0.58%, 78.60%+/-0.77%, P less than 0.05). Xingnaojing injection also reduced the leakage of BBB as compared with TBI group (15.11 microgram+/-0.63 microgram, 13.62 microgram+/-0.85 microgram, 10.06microgram+/-0.67 microgram, 9.54 microgram+/-0.41 microgram, P less than 0.05).
Xingnaojing injection could alleviate cerebral edema following TBI via reducing permeability of BBB.
探讨醒脑静注射液对创伤性脑损伤（TBI）大鼠脑水肿及血脑屏障（BBB）的影响。
选取108只成年雄性Sprague-Dawley大鼠作为研究对象，随机分为三组：假手术组、TBI组和醒脑静注射液组（10 ml/kg/d，腹腔注射）。采用改良的Feeney自由落体模型装置，以600 g.cm的冲击力建立大鼠TBI模型。分别于术后1、3、5和7天测量脑组织含水量及以伊文思蓝含量表示的BBB通透性。
假手术组脑组织含水量和伊文思蓝含量分别为78.97%±1.22%和5.13 μg±0.71 μg。TBI后，术后1、3、5和7天脑组织含水量显著增加（83.49%±0.54%、82.74%±0.72%、80.22%±0.68%、79.21%±0.60%），显著高于假手术组（P<0.05）。TBI组伊文思蓝含量较假手术组增加（16.54 μg±0.60 μg、14.92 μg±0.71 μg、12.44 μg±0.92 μg、10.14 μg±0.52 μg，P<0.05）。醒脑静注射液治疗后，与TBI组相比脑组织含水量降低（81.91%±1.04%、80.38%±0.72%、79.54%±0.58%、78.60%±0.77%，P<0.05）。与TBI组相比，醒脑静注射液还减少了BBB的渗漏（15.11 μg±0.63 μg、13.62 μg±0.85 μg、10.06 μg±0.67 μg、9.54 μg±0.41 μg，P<0.05）。
醒脑静注射液可通过降低BBB通透性减轻TBI后的脑水肿。
