采用蛋白质组学技术鉴定水稻叶鞘中赤霉素应答蛋白。

Identification of gibberellin acid-responsive proteins in rice leaf sheath using proteomics.

机构信息

The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, PR China.

出版信息

Front Biosci (Landmark Ed). 2010 Jun 1;15(3):826-39. doi: 10.2741/3648.

DOI:10.2741/3648

PMID:20515728

Abstract

The phytohormone gibberellin acid (GA) controls many aspects of plant development. In this study, we identified proteins that are differentially expressed between the rice (Oryza sativa L.) GA-deficient cultivar, Aijiaonante, and its parental line, Nante. Proteins were extracted from rice leaf sheath and examined by 2DGE. Among more than 1200 protein spots reproducibly detected on each gel, 29 were found to be highly up-regulated by GAs in Nante, and 6 were down-regulated by GAs in Aijiaonante. These 35 proteins were identified by MALDI-TOF MS and were classified into three groups based on their putative function in metabolism, stress/defense processes and signal transduction. These data suggest that metabolic pathways are the main target of regulation by GAs during rice development. Our results provide new information about the involvement of GAs in rice development.

摘要

植物激素赤霉素（GA）控制着植物发育的许多方面。在这项研究中，我们鉴定了在水稻（Oryza sativa L.）GA 缺陷型品种矮脚南特与其亲本品种南京 11 之间差异表达的蛋白质。从水稻叶鞘中提取蛋白质，并通过 2DGE 进行检查。在每个凝胶上可重复性检测到的 1200 多个蛋白质斑点中，有 29 个在南京 11 中被 GA 高度上调，而 6 个在矮脚南特中被 GA 下调。通过 MALDI-TOF MS 鉴定了这 35 个蛋白质，并根据它们在代谢、应激/防御过程和信号转导中的潜在功能将它们分为三组。这些数据表明，代谢途径是 GA 在水稻发育过程中主要的调控靶标。我们的结果提供了关于 GA 参与水稻发育的新信息。