Li Li, Wu Qiong, Wang Youyong, Aghdam Morteza Soleimani, Ban Zhaojun, Zhang Xiaochen, Lu Hongyan, Li Dong, Yan Jiawei, Limwachiranon Jarukitt, Luo Zisheng
Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, Zhejiang Key Laboratory for Agro-Food Processing, College of Biosystems Engineering and Food Science, Zhejiang University Hangzhou 310058 China
Ningbo Research Institute, Zhejiang University Ningbo 315100 China.
RSC Adv. 2019 May 7;9(25):14093-14108. doi: 10.1039/c9ra00549h.
Profound metabolic and proteomic changes involved in the primary and the secondary metabolism are required for the ripeness of fleshy fruit such as strawberries ( × ). Here we present the quantitative proteomic profiling in parallel with metabolic and transcriptional profiling at five developmental stages of strawberry fruit ripening, and correlations between changes in representative metabolites and the abundance of related proteins were analyzed. Hierarchical clustering analysis of the quantitative proteomic profiling identified 143 proteins in strawberry fruit across five developmental stages. Meanwhile, both protein abundance and gene expression spanned a wide range of roles, such as the primary and the secondary metabolism, defense system, and response to stress stimuli. The decreased abundance of proteins contributed to the carbohydrate metabolism and the up-regulated expression of secondary biosynthetic proteins was found to be positively correlated with the accumulation of primary and secondary metabolites during strawberry development. Moreover, with the same annotations and high homology, the gene function of key genes involved in primary and secondary metabolism (, , and ) was confirmed in the transient expression assay, which provides further evidence for the role of those genes in metabolism of strawberry fruit. The results of the present study may serve as an important resource for the functional analysis of the proteome and offer new perspectives on regulation of fruit quality.
像草莓（×）这样的肉质果实成熟需要初级和次级代谢过程中发生深刻的代谢和蛋白质组学变化。在此，我们展示了在草莓果实成熟的五个发育阶段，与代谢和转录谱分析并行的定量蛋白质组学分析，并分析了代表性代谢物变化与相关蛋白质丰度之间的相关性。对定量蛋白质组学分析进行层次聚类分析，确定了草莓果实五个发育阶段的143种蛋白质。同时，蛋白质丰度和基因表达涵盖了广泛的作用，如初级和次级代谢、防御系统以及对压力刺激的反应。在草莓发育过程中，发现参与碳水化合物代谢的蛋白质丰度降低，而次级生物合成蛋白质的表达上调与初级和次级代谢物的积累呈正相关。此外，通过相同注释和高度同源性，在瞬时表达试验中证实了参与初级和次级代谢的关键基因（，，和）的基因功能，这为这些基因在草莓果实代谢中的作用提供了进一步证据。本研究结果可能成为蛋白质组功能分析的重要资源，并为果实品质调控提供新的视角。
