Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Cold Spring Harb Perspect Biol. 2010 Jun;2(6):a003442. doi: 10.1101/cshperspect.a003442. Epub 2010 May 5.
Electron cryotomography (ECT) is an emerging technology that allows thin samples such as macromolecular complexes and small bacterial cells to be imaged in 3-D in a nearly native state to "molecular" ( approximately 4 nm) resolution. As such, ECT is beginning to deliver long-awaited insight into the positions and structures of cytoskeletal fi laments, cell wall elements, motility machines, chemoreceptor arrays, internal compartments, and other ultrastructures. This article describes the technique and summarizes its contributions to bacterial cell biology. For comparable recent reviews, see (Subramaniam 2005; Jensen and Briegel 2007; Murphy and Jensen 2007; Li and Jensen 2009). For reviews on the history, technical details, and broader application of electron tomography in general, see for example (Subramaniam and Milne 2004; Lucić et al. 2005; Leis et al. 2008; Midgley and Dunin-Borkowski 2009).
电子冷冻断层成像术（ECT）是一种新兴技术，它可以将大分子复合物和小细菌细胞等薄样品在近天然状态下进行三维成像，达到“分子”（约 4nm）分辨率。因此，ECT 开始为细胞骨架丝、细胞壁成分、运动机器、化学感受器阵列、内部隔室和其他超微结构的位置和结构提供了期待已久的见解。本文描述了该技术，并总结了它对细菌细胞生物学的贡献。有关类似的最新评论，请参见（Subramaniam 2005；Jensen 和 Briegel 2007；Murphy 和 Jensen 2007；Li 和 Jensen 2009）。有关电子断层扫描技术的历史、技术细节以及更广泛应用的一般评论，请参见例如（Subramaniam 和 Milne 2004；Lucić 等人，2005；Leis 等人，2008；Midgley 和 Dunin-Borkowski 2009）。
