Schwab Birgit, Hülskamp Martin
Cold Spring Harb Protoc. 2010 Jun;2010(6):pdb.prot4953. doi: 10.1101/pdb.prot4953.
For almost 100 years, neutral red has been used to stain living cells and fixed tissue. It can be used as a general-purpose stain, a pH indicator (turning from red to yellow, as the medium becomes alkaline), or a nuclear stain. In this protocol, neutral red is used to stain plant vacuoles.
近100年来,中性红一直用于对活细胞和固定组织进行染色。它可用作通用染色剂、pH指示剂(随着培养基变为碱性,颜色从红色变为黄色)或细胞核染色剂。在本实验方案中,中性红用于对植物液泡进行染色。